Do you know a protocol to extract DNA from Gram + bacteria in human feces with magnetic beads technology?

I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.

08 September 2014 5,860 5 View

Can anyone tell me how a binding buffer for magnetic beads is made?

I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.

03 April 2014 1,169 2 View

Can anyone help me with miRNA extraction with magnetic beads?

I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks

02 March 2014 5,143 0 View

Can a pair of primer designed for a specific cDNA amplify also a genomic region?

In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region

01 February 2014 430 19 View

Are RNA viroids from plants studied like other viruses or do they need other strategies?

RNA viroids studies.

01 February 2014 5,915 2 View

What are the methods for evaluating methylation conversion?

Is a simple PCR sufficient?

31 December 2013 4,305 2 View

How is lysis buffer for DNA extraction made?

I would like to make my own lysis buffer for DNA extraction from blood and tissues

10 November 2013 7,472 3 View

What is the average yield (ng/ul) after a DNA extraction using magnetic beads starting from plant samples (example, 10 mg of leaf tissue)?

I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.

10 November 2013 4,657 2 View

I would like to know if 1,2% gel is good for running the genomic DNA. Can you help me?

In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.

09 October 2013 9,292 8 View

Have you ever tried the TATAA Universal DNA spike kit to detect any contaminants in your genomic DNA sample?

The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?

09 October 2013 8,269 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View