I would like to make my own lysis buffer for DNA extraction from blood and tissues
Hi check the attachment
thanks a lot, Yinkuse!
Thank you so much, Merlin!
I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.
08 September 2014 5,860 5 View
I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.
03 April 2014 1,169 2 View
I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks
02 March 2014 5,143 0 View
It is sufficient to remove the tissue from RNAlater and put it in lysis solution?
02 March 2014 5,905 2 View
In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region
01 February 2014 430 19 View
RNA viroids studies.
01 February 2014 5,915 2 View
Is a simple PCR sufficient?
31 December 2013 4,305 2 View
I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.
10 November 2013 4,657 2 View
In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.
09 October 2013 9,292 8 View
The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?
09 October 2013 8,269 0 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View