I would like to make my own lysis buffer for DNA extraction from blood and tissues
Hi check the attachment
thanks a lot, Yinkuse!
Thank you so much, Merlin!
I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.
08 September 2014 5,964 5 View
I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.
03 April 2014 1,336 2 View
I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks
02 March 2014 5,221 0 View
It is sufficient to remove the tissue from RNAlater and put it in lysis solution?
02 March 2014 5,990 2 View
In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region
01 February 2014 524 19 View
RNA viroids studies.
01 February 2014 6,005 2 View
Is a simple PCR sufficient?
31 December 2013 4,397 2 View
I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.
10 November 2013 4,871 2 View
The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?
09 October 2013 8,348 0 View
In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.
09 October 2013 9,395 8 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View