If I want to clearly define the growth phase from a grwoth curve obtained from mammalian cell batch culture, what scale should I apply to the curve?

As mentioned above, I just conducted a batch culture of a mammalian cell line, and I got the daily viable cell density. But I'm a little bit confused about how to define its growth phase from its...

08 September 2017 6,726 0 View

Does CHO cell line considered as cancer cell line?

05 June 2017 8,091 1 View

What is the proper format to list the describe the material used in article?

What is the proper format to list the describe the material used in article?

05 June 2017 350 0 View

Can anyone kindly provide some pictures about how the CHO cell culture looks like when it is contaminated?

I'm currently culturing the CHO cell in 125-mL shake flask. Some of the cultures had the trend that the viability keep decreasing. But I'm not sure about the reason, but it may be some...

04 May 2017 976 3 View

Does disodium hydrogen phosphate anhydrous can be used to substitute for disodium hydrogen phosphate dodecahydrate when used for yeast medium?

As mentioned above

04 May 2017 2,054 2 View

About the 75% ethanol used in RNA extraction with Trizol, is 75% the volume precentage or weight percentage?

As what I describe in the question, I'm confused by this 75% ethanol. As I can see, in most of the protocol online, only 75% ethanol is mentioned, without stating if it's volume percentage or...

04 May 2017 8,103 0 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View