As what I describe in the question, I'm confused by this 75% ethanol. As I can see, in most of the protocol online, only 75% ethanol is mentioned, without stating if it's volume percentage or weight percentage.
As mentioned above, I just conducted a batch culture of a mammalian cell line, and I got the daily viable cell density. But I'm a little bit confused about how to define its growth phase from its...
08 September 2017 6,811 0 View
According to the definition on www.cancer.gov, cancer cell lines refer to cancer cells that keep dividing and growing over time, under certain conditions in a laboratory. It seems that CHO cells...
05 June 2017 8,253 1 View
Are there any criteria that what percentage of GC can be regarded as GC-rich in PCR primer design?
05 June 2017 1,817 3 View
What is the proper format to list the describe the material used in article?
05 June 2017 547 0 View
I'm currently culturing the CHO cell in 125-mL shake flask. Some of the cultures had the trend that the viability keep decreasing. But I'm not sure about the reason, but it may be some...
04 May 2017 1,129 3 View
As mentioned above
04 May 2017 2,188 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View