If I want to clearly define the growth phase from a grwoth curve obtained from mammalian cell batch culture, what scale should I apply to the curve?

As mentioned above, I just conducted a batch culture of a mammalian cell line, and I got the daily viable cell density. But I'm a little bit confused about how to define its growth phase from its...

08 September 2017 6,726 0 View

Does CHO cell line considered as cancer cell line?

05 June 2017 8,091 1 View

Is there any criteria that what precentage of GC can be regarded as GC-rich in PCR primer design?

Are there any criteria that what percentage of GC can be regarded as GC-rich in PCR primer design?

05 June 2017 1,667 3 View

What is the proper format to list the describe the material used in article?

What is the proper format to list the describe the material used in article?

05 June 2017 350 0 View

Can anyone kindly provide some pictures about how the CHO cell culture looks like when it is contaminated?

I'm currently culturing the CHO cell in 125-mL shake flask. Some of the cultures had the trend that the viability keep decreasing. But I'm not sure about the reason, but it may be some...

04 May 2017 976 3 View

About the 75% ethanol used in RNA extraction with Trizol, is 75% the volume precentage or weight percentage?

As what I describe in the question, I'm confused by this 75% ethanol. As I can see, in most of the protocol online, only 75% ethanol is mentioned, without stating if it's volume percentage or...

04 May 2017 8,103 0 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How the standard hydrogen electrode value is convertible into reversible hydrogen electrode?

As much I remember +0.0592*pH, as I am working on CatMAP, so my voltage values are in SHE, not RHE.

18 February 2021 1,685 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

Can explain this diagram to me, is this the average consumption curve for one day or the total consumption of days in a month summed up in specific?

The hourly load curves of the selected household in each season are shown in Fig. 28. Figure 28 shows the demand for electricity in four months on an hourly basis. Can you explain this diagram to...

08 February 2021 657 16 View