If I want to clearly define the growth phase from a grwoth curve obtained from mammalian cell batch culture, what scale should I apply to the curve?

As mentioned above, I just conducted a batch culture of a mammalian cell line, and I got the daily viable cell density. But I'm a little bit confused about how to define its growth phase from its...

08 September 2017 6,726 0 View

Does CHO cell line considered as cancer cell line?

05 June 2017 8,091 1 View

Is there any criteria that what precentage of GC can be regarded as GC-rich in PCR primer design?

Are there any criteria that what percentage of GC can be regarded as GC-rich in PCR primer design?

05 June 2017 1,667 3 View

Can anyone kindly provide some pictures about how the CHO cell culture looks like when it is contaminated?

I'm currently culturing the CHO cell in 125-mL shake flask. Some of the cultures had the trend that the viability keep decreasing. But I'm not sure about the reason, but it may be some...

04 May 2017 976 3 View

Does disodium hydrogen phosphate anhydrous can be used to substitute for disodium hydrogen phosphate dodecahydrate when used for yeast medium?

As mentioned above

04 May 2017 2,054 2 View

About the 75% ethanol used in RNA extraction with Trizol, is 75% the volume precentage or weight percentage?

As what I describe in the question, I'm confused by this 75% ethanol. As I can see, in most of the protocol online, only 75% ethanol is mentioned, without stating if it's volume percentage or...

04 May 2017 8,103 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View