Can anyone can give me a clue, which cell line expresses the 17B HSD3 prominently?

More R M Bazai's questions See All

Method development of paracetamol oral suspension ?

Hi Dear Actually, i am trying to develop the related substances method for paracetamol oral suspension in RP HPLC and i am getting small peak in the diluent at the RT time of paracetamol. I run...

02 March 2019 4,373 3 View

Force degdradation for pharmacutical product in method developmenet?

can anyone suggest as I cam across different paper doing the acidic and basic and stress oxidation for 60minuties on top bench and some leave for 7 days. Can anyone please suggest why there is...

10 November 2018 4,245 0 View

Capacity Factor in HPLC chromatography?

can any one share their expertise in Capacity Factor, I developed the impurities method and the asymmetry is about 1.0 for all the impurities and now I have been asked it should be more than 2 the...

10 November 2018 8,142 0 View

Does anyone help in paracetamol impurities method?

I am trying to develop method for paracetamol impurities in oral suspension,at the moment I am using 20mM phosphate buffer but everytime the same mobile phase and same solution give me different...

10 November 2018 6,782 0 View

Can any one please tell me which other statistics can be used for IC50?

Hi Can anyone explain ,which other software or statistics can be used for determination of IC50 other than using Excel.

04 May 2016 5,255 2 View

How can I increase the expression of desired receptors?

Does anyone have any idea if estrogen (hormone) is fed to cell for certain amount of weeks and will it increase the expression of estrogen receptors (ER)? And can anyone send me paper for similar...

02 March 2015 3,320 2 View

Formic acid or tritiated water?

I was wondering that Aromatase assay is called tritiated water release assay and number of theories been proposed for release of formic acid and tritiated water release. does any knows that what...

11 December 2014 3,090 0 View

Can any one help with TLC mobile phase of androstanedione?

Since, I do not have standard steroid (Androstanedione), I was wondering that if any one can tell me what is the TLC mobile phase for androstanedione and is it UV-active or not. I want to check...

11 December 2014 576 3 View

Does any one know which cell line expresses 5-reductase enzyme?

Does any know whuch prostate cancer cell line express 5a-reductase enzymes

11 December 2014 7,533 3 View

Can anyone knows why highly fat content food is risk of cancer, what rational is involved?

Fatty food

10 November 2014 3,758 0 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View