Method development of paracetamol oral suspension ?

Hi Dear Actually, i am trying to develop the related substances method for paracetamol oral suspension in RP HPLC and i am getting small peak in the diluent at the RT time of paracetamol. I run...

02 March 2019 4,276 3 View

Force degdradation for pharmacutical product in method developmenet?

can anyone suggest as I cam across different paper doing the acidic and basic and stress oxidation for 60minuties on top bench and some leave for 7 days. Can anyone please suggest why there is...

10 November 2018 4,157 0 View

Capacity Factor in HPLC chromatography?

can any one share their expertise in Capacity Factor, I developed the impurities method and the asymmetry is about 1.0 for all the impurities and now I have been asked it should be more than 2 the...

10 November 2018 8,066 0 View

Can any one please tell me which other statistics can be used for IC50?

04 May 2016 5,182 2 View

How can I increase the expression of desired receptors?

Does anyone have any idea if estrogen (hormone) is fed to cell for certain amount of weeks and will it increase the expression of estrogen receptors (ER)? And can anyone send me paper for similar...

02 March 2015 3,193 2 View

Formic acid or tritiated water?

11 December 2014 3,009 0 View

Can any one help with TLC mobile phase of androstanedione?

Since, I do not have standard steroid (Androstanedione), I was wondering that if any one can tell me what is the TLC mobile phase for androstanedione and is it UV-active or not. I want to check...

11 December 2014 484 3 View

Does any one know which cell line expresses 5-reductase enzyme?

Does any know whuch prostate cancer cell line express 5a-reductase enzymes

11 December 2014 7,447 3 View

Can anyone knows why highly fat content food is risk of cancer, what rational is involved?

Fatty food

10 November 2014 3,678 0 View

Can anyone can give me a clue, which cell line expresses the 17B HSD3 prominently?

Hi I wondering if anyone can give me a clue, which cell line expresses the 17B HSD3 prominently. Please.

10 November 2014 1,833 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View