can any one share their expertise in Capacity Factor, I developed the impurities method and the asymmetry is about 1.0 for all the impurities and now I have been asked it should be more than 2 the Capacity Factor. please help
Hi Dear Actually, i am trying to develop the related substances method for paracetamol oral suspension in RP HPLC and i am getting small peak in the diluent at the RT time of paracetamol. I run...
02 March 2019 4,373 3 View
can anyone suggest as I cam across different paper doing the acidic and basic and stress oxidation for 60minuties on top bench and some leave for 7 days. Can anyone please suggest why there is...
10 November 2018 4,245 0 View
I am trying to develop method for paracetamol impurities in oral suspension,at the moment I am using 20mM phosphate buffer but everytime the same mobile phase and same solution give me different...
10 November 2018 6,782 0 View
Hi Can anyone explain ,which other software or statistics can be used for determination of IC50 other than using Excel.
04 May 2016 5,255 2 View
Does anyone have any idea if estrogen (hormone) is fed to cell for certain amount of weeks and will it increase the expression of estrogen receptors (ER)? And can anyone send me paper for similar...
02 March 2015 3,320 2 View
I was wondering that Aromatase assay is called tritiated water release assay and number of theories been proposed for release of formic acid and tritiated water release. does any knows that what...
11 December 2014 3,090 0 View
Since, I do not have standard steroid (Androstanedione), I was wondering that if any one can tell me what is the TLC mobile phase for androstanedione and is it UV-active or not. I want to check...
11 December 2014 576 3 View
Does any know whuch prostate cancer cell line express 5a-reductase enzymes
11 December 2014 7,533 3 View
Fatty food
10 November 2014 3,758 0 View
Hi I wondering if anyone can give me a clue, which cell line expresses the 17B HSD3 prominently. Please.
10 November 2014 1,914 2 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View