Does any know whuch prostate cancer cell line express 5a-reductase enzymes
Have a look on Genecards.org
No i will check it but most of the paper says LNCAP and DU145 but i could not see conversion in LNCAP using T as substrate.
LNcAP definitely express 5 alpha reductase
Hi Dear Actually, i am trying to develop the related substances method for paracetamol oral suspension in RP HPLC and i am getting small peak in the diluent at the RT time of paracetamol. I run...
02 March 2019 4,373 3 View
can anyone suggest as I cam across different paper doing the acidic and basic and stress oxidation for 60minuties on top bench and some leave for 7 days. Can anyone please suggest why there is...
10 November 2018 4,245 0 View
can any one share their expertise in Capacity Factor, I developed the impurities method and the asymmetry is about 1.0 for all the impurities and now I have been asked it should be more than 2 the...
10 November 2018 8,142 0 View
I am trying to develop method for paracetamol impurities in oral suspension,at the moment I am using 20mM phosphate buffer but everytime the same mobile phase and same solution give me different...
10 November 2018 6,782 0 View
Hi Can anyone explain ,which other software or statistics can be used for determination of IC50 other than using Excel.
04 May 2016 5,255 2 View
Does anyone have any idea if estrogen (hormone) is fed to cell for certain amount of weeks and will it increase the expression of estrogen receptors (ER)? And can anyone send me paper for similar...
02 March 2015 3,320 2 View
I was wondering that Aromatase assay is called tritiated water release assay and number of theories been proposed for release of formic acid and tritiated water release. does any knows that what...
11 December 2014 3,090 0 View
Since, I do not have standard steroid (Androstanedione), I was wondering that if any one can tell me what is the TLC mobile phase for androstanedione and is it UV-active or not. I want to check...
11 December 2014 576 3 View
Fatty food
10 November 2014 3,758 0 View
Hi I wondering if anyone can give me a clue, which cell line expresses the 17B HSD3 prominently. Please.
10 November 2014 1,914 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
