Is it reasonable to use half your dataset to carry out exploratory analysis leading to specific hypotheses, and then test these using the other half?

There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...

25 July 2019 9,588 4 View

How to add published research directly from the publication web site?

I have published articles and a PhD thesis which are not listed here. How can I add the link to the publication site here?

17 May 2019 4,398 2 View

What is the experience with Duck Duck Go as a search engine?

Concern about data mining and the uses to which the mined data are put suggest that privacy is not just a matter of personal sensitivities. It may, at least partially, be a social duty if it can...

15 September 2018 6,988 4 View

How does taking multiple measurements under the same condition affect experiment power and effect sizes?

In our research group we carry out psychophysics experiments that involve taking multiple measurements, e.g. reaction times or correct detections, under the same combination of variables. I...

01 August 2018 7,372 7 View

Is there a source to learn which cell lines bind RGD (are integrin-expressing)?

Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...

25 March 2018 8,053 9 View

Can anyone suggest a way to quantify viral RNA in the pg range other than PCR or Agilent's technology?

Viral genome is too large for Agilent's technology, and I do not want to use RT-qPCR. I am thinking mass spec, but would rather get something I can do in our labs.

08 March 2018 192 3 View

Looking for help building a layered structure in FDTD

I'm looking to build a layered quarter wave stack structure in a FDTD simulation. I've tried both 2-D and 3-D and it hasn't worked. I'm able to do a block of solid material but not layers. The...

10 August 2017 6,385 13 View

Is it appropriate to use a weighted sample size in calculating standard errors and conducting significance tests?

SPSS' regression procedure, when conducting a weighted analysis (to provide unbiased estimates of population parameters) bases standard errors and significance tests on the weighted N. For a...

02 January 2017 4,722 4 View

I'm trying to model choices over time. Does SPSS have a statistical procedure for this analysis?

This administrative study investigates the choices students make in a menu of choices curriculum design. Given a gateway course, where do they go from there? And then from there, etc.? I will have...

10 September 2015 4,881 7 View

How can I use an Instron for soil penetration resistance measurements?

12 June 2015 6,962 3 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View