Explants having mucilage are challenging to eliminate the contamination due to the formation of gelatinous layer around the explant which reduces the effectiveness of the sterilizing agents.
Gaurab, try cutting your explants in a solution of antioxidants such as citric acid. Also try to submerge the explants in solution during preparation. Finally, replace cutting materials (e.g., blades) regularly. If explants are from the field, try changing the season of sampling (so younger tissue in rapid growth will be less contaminated than older tissue in a dormant stage). Contact me for the exchange of more ideas if you need. The stringency of treatments often depends on the plant material.
Hi Gaurab,
I suggest you steralize your explants a little bit longer (e.g in 2% bleach), or add some antibiotics (e.g 150 mg/l timentin).
I also agree Jaime's suggestion: cut your explants in citric acid (or antibiotic ) solution.
Good luck!
I don't understand the question but I am curious. Could you precise if the explant is mucilaginous before of after excision? Before of after sterilisation?
Dear Mr Gogoi
You may culture the motherland in the greenhouse and will reduce contamination
When a plant material is hairy (apple twigs) or sticky (Alnus glutinosa), I use a 2-steps procedure for the sterilisation. Is is described in my paper about micropropagation of seven Alnus species.
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