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What are the substitutes to Sodium azide and EMS as cytotoxic agents?
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Explants having mucilage are challenging to eliminate the contamination due to the formation of gelatinous layer around the explant which reduces the effectiveness of the sterilizing agents.
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During the rooting of Plant Tissue culture the developed shoots are cultured in MS half strength with different growth additives.
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Moisture and water droplets starts to accumulate at the inner wall of the culture tubes/bottles, which is an inconvenience in observation and taking photographs.
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There is often a variations of concentration of PGR required for the in vitro culture of plants. Some plants may require very low concentration whereas other may require higher concentration. So...
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Germination seed is often a good choice for regenerating plant in vitro. But in my experiment I have failed which only ends up in some kind of colorless tissue with no any significance changes or...
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The main expenses incurred in plant tissue culture is the sucrose and agar. Therefore there is always a search for a cost effective solution for the same as plant tissue culture needs numerous...
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