my gene of interest size is about 4kb. I used hi fidelity taq pol as well as Q5 from NEB but still its happening. my forward primer composed of a 4 adenosine then restriction site followed by 6x his tag then enterokinase site and forward primer of amplicon.(63 bases) and reverse is normal,4 adenosine then restriction site followed by reverse primer of amplicon( 30bases). i changed all the reagents to improve pcr but same result follows. what should I do now?

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