454 sequencing: sRNA's in bacteria

12 December 2008 0 1K Report

Hi,

We are planning to do a pyrosequencing run for transcriptomic analysis of a bacterium, and I am really hoping that we'll also catch some sRNA's, but I am not very optimistic, since these are really short -20-30 bases- and I don't even know if they'll be reverse transcribed or sequenced... Does anyone have an idea?

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Science method

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