18 Questions 35 Answers 0 Followers
Questions related from Basak Ozturk
Hi, I have a friend who desperately needs some nice ideas about a problem... He has some weird bacteria who are normally susceptible to Chloramphenicol, but he was able to isolate some...
10 October 2010 3,626 1 View
Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.
08 August 2009 4,184 0 View
Hey, I wanted to test the RNA dependent RNA polymerase from NEB for a protocol I'm working on, it is a cool enzyme that produces dsRNA from ssRNA without need of any primer. The bad thing is,...
07 July 2009 9,290 0 View
Hi, I have been isolating some plasmids from E. coli for routine use (like PBBR1MCS), but I have a problem accurately quantifying the DNA that I get.. I used the Qiagen miniprep columns for 5...
07 July 2009 6,020 0 View
Hey, I am now trying to develop genetic systems for a bacterium, however it is pretty difficult to get DNA inside them somehow... I read the following article on Ultrasound mediated DNA (and...
06 June 2009 8,872 0 View
Dear i am work on RT-PCR, i have 2 bands on agarose gel (one at 150 BP and other at 400 BP), the 2 bands are parts from my gen after sequencing but now, i need to transfer all my product for form...
06 June 2009 9,972 0 View
Hi, I have to perform an experiment to see if my bacteria have strong restriction enzymes that are degrading the plasmids I am giving them... for that we had the idea to extract the bacterial...
05 May 2009 5,097 0 View
Would it be possible to use the Exonuclease I to remove unbound primers from a reverse-transcription reaction mix? I thought it might be suitable since cDNA and RNA are existing in a complex. Or...
04 April 2009 10,001 0 View
Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...
03 March 2009 3,306 7 View
Hi, I know, this is a very basic question but it just comes up again and again... Which method do you actually use for cleaning-up PCR products? I always have the impression that when I use the...
03 March 2009 8,935 3 View
Hi, Does anyone have an idea how to run ssDNA on agarose gels? I tried my regular protocol, but I am suspecting that the ssDNA is forming some secondary structures..
03 March 2009 462 1 View
Hi, I have recently been a bit confused by the T4 RNA ligases... I know they are being used for 5' and 3' RACE, and also for RNA end labeling... Does anyone know if I can also use them to tag...
02 February 2009 1,066 1 View
Check RTPrimerDB (http://medgen.ugent.be/rtprimerdb), PrimerBank (http://pga.mgh.harvard.edu/primerbank/index.html) and Real Time PCR Primer Sets (http://www.realtimeprimers.org) for suitable,...
02 February 2009 1,758 0 View
Hi, there was recently a question about real time results and how to interpret them. I would recommend the paper: Quantification of mRNA using real-time RT-PCR, from Nolan et al., Nature...
02 February 2009 2,972 1 View
Hi, I have been using the SuperScript kit to reverse transcribe some polyA tailed mRNA... the strange thing is after purification with RNA hydrolization and precipitation, the amount of cDNA...
01 January 2009 8,934 0 View
Hi, We are planning to do a pyrosequencing run for transcriptomic analysis of a bacterium, and I am really hoping that we'll also catch some sRNA's, but I am not very optimistic, since these...
12 December 2008 5,955 1 View
Hi, I have been trying to reverse transcribe total bacterial RNA by polyadenylation and oligod(T) primers... I used the polyA polymerase kit from Epicentre, cleaned up the reaction on spin...
12 December 2008 6,251 5 View
12 December 2008 1,306 0 View
