Hi, I have a friend who desperately needs some nice ideas about a problem... He has some weird bacteria who are normally susceptible to Chloramphenicol, but he was able to isolate some...
09 October 2010 3,572 1 View
Hey, I wanted to test the RNA dependent RNA polymerase from NEB for a protocol I'm working on, it is a cool enzyme that produces dsRNA from ssRNA without need of any primer. The bad thing is,...
06 July 2009 9,235 0 View
Hi, I have been isolating some plasmids from E. coli for routine use (like PBBR1MCS), but I have a problem accurately quantifying the DNA that I get.. I used the Qiagen miniprep columns for 5...
06 July 2009 5,964 0 View
Hey, I am now trying to develop genetic systems for a bacterium, however it is pretty difficult to get DNA inside them somehow... I read the following article on Ultrasound mediated DNA (and...
05 June 2009 8,824 0 View
Dear i am work on RT-PCR, i have 2 bands on agarose gel (one at 150 BP and other at 400 BP), the 2 bands are parts from my gen after sequencing but now, i need to transfer all my product for form...
05 June 2009 9,925 0 View
Hi, I have to perform an experiment to see if my bacteria have strong restriction enzymes that are degrading the plasmids I am giving them... for that we had the idea to extract the bacterial...
04 May 2009 5,049 0 View
Would it be possible to use the Exonuclease I to remove unbound primers from a reverse-transcription reaction mix? I thought it might be suitable since cDNA and RNA are existing in a complex. Or...
03 April 2009 9,952 0 View
Hi, Does anyone have an idea how to run ssDNA on agarose gels? I tried my regular protocol, but I am suspecting that the ssDNA is forming some secondary structures..
02 March 2009 403 1 View
Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...
02 March 2009 3,255 7 View
Hi, I know, this is a very basic question but it just comes up again and again... Which method do you actually use for cleaning-up PCR products? I always have the impression that when I use the...
02 March 2009 8,879 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...
20 February 2021 6,897 1 View
I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...
15 February 2021 7,444 2 View
I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...
14 February 2021 7,800 7 View
I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...
09 February 2021 8,594 3 View
In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...
08 February 2021 2,792 1 View
Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...
07 February 2021 9,530 3 View
I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...
04 February 2021 1,509 3 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View
