Hi, I have a friend who desperately needs some nice ideas about a problem... He has some weird bacteria who are normally susceptible to Chloramphenicol, but he was able to isolate some...
09 October 2010 3,670 1 View
Hey, I wanted to test the RNA dependent RNA polymerase from NEB for a protocol I'm working on, it is a cool enzyme that produces dsRNA from ssRNA without need of any primer. The bad thing is,...
06 July 2009 9,330 0 View
Hi, I have been isolating some plasmids from E. coli for routine use (like PBBR1MCS), but I have a problem accurately quantifying the DNA that I get.. I used the Qiagen miniprep columns for 5...
06 July 2009 6,061 0 View
Hey, I am now trying to develop genetic systems for a bacterium, however it is pretty difficult to get DNA inside them somehow... I read the following article on Ultrasound mediated DNA (and...
05 June 2009 8,910 0 View
Dear i am work on RT-PCR, i have 2 bands on agarose gel (one at 150 BP and other at 400 BP), the 2 bands are parts from my gen after sequencing but now, i need to transfer all my product for form...
05 June 2009 10,010 0 View
Hi, I have to perform an experiment to see if my bacteria have strong restriction enzymes that are degrading the plasmids I am giving them... for that we had the idea to extract the bacterial...
04 May 2009 5,143 0 View
Would it be possible to use the Exonuclease I to remove unbound primers from a reverse-transcription reaction mix? I thought it might be suitable since cDNA and RNA are existing in a complex. Or...
03 April 2009 10,030 0 View
Hi, Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong... And, supposedly the...
02 March 2009 3,353 7 View
Hi, Does anyone have an idea how to run ssDNA on agarose gels? I tried my regular protocol, but I am suspecting that the ssDNA is forming some secondary structures..
02 March 2009 505 1 View
Hi, I know, this is a very basic question but it just comes up again and again... Which method do you actually use for cleaning-up PCR products? I always have the impression that when I use the...
02 March 2009 8,984 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View
