Hi,

I have to perform an experiment to see if my bacteria have strong restriction enzymes that are degrading the plasmids I am giving them... for that we had the idea to extract the bacterial proteins and incubate them with the plasmid, to see if it still stays as it was or if it's digested... the problem is that most extraction methods I have include cell lysis with urea and detergents, as well as grinding or sonication and I really don't think that the enzymes will still stay intact after such a procedure.. On the other hand I guess the DNAases will also still be there.

Any ideas to extract potentially active proteins from bacteria? Or is it just not feasible?

Thanks!

b.