Phi6 RNA polymerase

07 July 2009 0 9K Report

Hey,

I wanted to test the RNA dependent RNA polymerase from NEB for a protocol I'm working on, it is a cool enzyme that produces dsRNA from ssRNA without need of any primer. The bad thing is, after setting up the reaction and purifying it on MinElute columns, I get no RNA! there is nothing, at all... I thought I had RNAase contamination, but I'm really careful, and there are RNAase inhibitors in the reaction as well...

Does any of you have experience with such a situation? I know, it's unusual...

thanks!!

basak

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