ssDNA electrophoresis

03 March 2009 1 475 Report

Hi,

Does anyone have an idea how to run ssDNA on agarose gels? I tried my regular protocol, but I am suspecting that the ssDNA is forming some secondary structures..

Stephen R. Doyle

Add 1M urea to your TAE buffer, and make the gel and running buffer with it. Then make some gel loading buffer (bromophenol blue etc.) with 8M urea. Add to sample, and then heat to 80deg Celsius for 10 mins before loading gel. Run the gel at 4deg. We do it in a cold room.

Works a treat!

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Science method

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