Dear

i am work on RT-PCR, i have 2 bands on agarose gel (one at 150 BP and other at 400 BP), the 2 bands are parts from my gen after sequencing but now, i need to transfer all my product for form band which at 400 BP at high amount and remove the band at 150 beacuse i haven't 3- race.

i made the C-DNA at 45 o for 45 min

and PCR- ( annealing at 60 o C for 1 min)

what can i do and what can i change, please?

saly