I am actually wondering what other safe tricks I could use to dissolved my plant extracts, having used distilled water and 100% DMSO to no avail? I have gone from 5mg/mL stock to 1mg/ML despite the fact that these extracts are to be tested on cancer cells. I really do not want to go more dilute with DMSO.
I could do with a good advise at this point, I really want to test these drugs - this is why I brought them to France all the way from Nigeria :D
Many thanks.
PS: The most notorious of these extracts are the metanol, n-hexane and ethylacetate ones albeit one aqueous is also surprisingly acting up!
Please have a look at this previous thread
https://www.researchgate.net/post/Method_for_dissolving_plant_extract_inn_DMSO
Some people are suggesting ethanol or methanol.
Here are some short suggestions for dissolving plant extracts:
1. Try different solvents such as ethanol, methanol, acetone, chloroform, or a mixture of these solvents.
2. Use a combination of solvents (co-solvent system) to enhance solubility.
3. Gently heat the solvent and plant extract mixture while stirring.
4. Consider using sonication to break down particles and enhance dissolution.
Depending on the research type, you can try the tween series.
Oludele John Olanlokun, Thanks my brother, Tween series are forbidden here in the lab, cos they are detergents that could interfere with mitochondria assays......
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