Usually, I use cDNA and perform gradient PCR for primer standardization. What kind of sample cDNA do I have to use in this case and what steps do I need to consider for standardization?
Dear sir/mam Greetings for the day ! (1). I am using quantum espresso(QE) to calculate band decomposed charge density. To my knowledge, the kpoint at which we want to calculate the charge...
21 August 2020 1,095 2 View
12 July 2020 6,555 1 View
How to prepare gas sensor device for nanofibers? I was prepared metal oxide nanofibers...how i can establish gas sensing device with this metal oxide nanofibers? Can you please help me
06 November 2019 1,712 3 View
Provide the properties and data sheet for Ti-3Cu alloy for biomedical applications ???
18 November 2018 3,704 1 View
whether titanium copper alloy can be used for bio medical application ? suggest any journals??
27 October 2018 4,021 2 View
i am trying to measure in four probe method for resitivity and band gap. how to prepare our samples for four probe technique.
25 April 2017 1,947 1 View
27 March 2017 4,238 3 View
I am currently looking at a text (1891) in which the above sentence appears. The complete para looks like this. "We may safely lay down the proposition that no one will deny the simple truth that...
06 February 2017 3,107 5 View
I have made predictive current control for dc to ac two level 3 phase. When doing THD analysis i get 8.39% whereas 5% is acceptable. How can i get the desired THD levels
19 October 2016 5,586 3 View
I have designed a controller using predictive control for NPC VSI 3 level . the out put result shows that there is shift in one phase of the three phases what could be the possible errors
25 May 2016 2,648 5 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...
01 March 2021 9,536 3 View
As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...
01 March 2021 3,199 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View