Usually, I use cDNA and perform gradient PCR for primer standardization. What kind of sample cDNA do I have to use in this case and what steps do I need to consider for standardization?
That forces are the particular atoms/molecules forces due the effect of another atoms/molecules
10 March 2024 5,465 3 View
I have a dataset of different taxa of five different site with respect to environment fluctuation? the top contributing taxa (sharing highest % of dissimilarity index ) is sensitive to...
28 January 2024 6,349 0 View
As of now, there is no public database available for this kind of sample to take as a control.
15 October 2023 6,908 3 View
I intend to perform cytokine measurements in rodent samples and would like to use the bead based cytokine multiplexing analysis, preferably using the Bio-PlexTM Pro Multiplex Immunoassays. If...
06 August 2023 4,118 2 View
Majorly used geometries for any storage purposes are cylindrical and spherical geometries depending on state of fluid, is there any other geometry which can be efficient than both the existing...
19 April 2023 6,433 0 View
Available N, Green gram.
18 February 2023 5,340 2 View
Can any body suggest how to calculate organic matter decomposition rate of soil or compost? is there any formula? please suggest some relevant references.
13 January 2023 2,659 4 View
If I want to do research on PCM using Molecular Dynamics can you give suggestion which alkane is the best one to find out mass and thermal transport properties and why? your help is very useful...
29 December 2022 9,032 1 View
I am trying to calculate the mean squared displacement and have a C-code in Molecular Dynamics Simulation. I am having a hard time in getting the exact plot as proposed for a particular system....
14 November 2022 9,841 1 View
14 November 2022 8,286 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View