How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

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23 February 2021 4,014 3 View

No streamlines present in a small region inside a flow. This means exactly what?

No streamlines present means whether the flow is getting obstructed there and the velocity inside the region is zero? Or else, the fluid is trapped inside that region with a nonzero velocity?...

22 February 2021 5,249 10 View

Low DNA yields with midiprep and maxiprep plasmid extraction kits but excellent results with miniprep kits. What can be a possible reason?

I need to extract large amounts of plasmid DNA for transfection into mammalian cell lines with as little as possible enterotoxins and I usually use the Qiagen Endofree plasmid maxi kit for the...

09 February 2021 3,779 6 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View