What are the DNA sequence of AAY ,GPGPG AND KK linkers?

09 October 2023 1 174 Report

Hi everyone, I need to find Nucleotide sequence of protein linker ( AAY , GPGPG and KK)

Hello Amjed,

We need to know the type of linker and for which purpose you need for. All of these factors will change the sequence. I did some cloning on the linker when I was doing CRISPR experiments.

Badges
Science topic

More Amjed Alsultan's questions See All

Hi, is any perfect tool for analysis of whole genome sequence of bacteria?

we made a whole genom sequence of some bacterial isolates and we need a pefect tool for analysis of fasta sequence

20 March 2023 4,195 2 View

What is the explanation of the presence of halide and nitrogen in XPS when preparing nickel nanoparticles?

Nickel nanoparticles were prepared, and when preparing nickel chloride was used in addition to hydrazine and other materials When examining the obtained nanoparticles by XPS , chlorine and...

08 January 2022 6,111 4 View

Is anyone familiar with DNA extraction from Manure ?

when we use commercial kit for DNA extraction from soil we find nothing , we try to improve lysis step but we fail .anyone have a suggestions or advises. thanks in advance

30 December 2021 5,980 5 View

CTAB capped silver nanoparticles to reduction 4-nitrophenol ?

Hello evreybody When we used CTAB as a capping agent in reducing 4-nitrophenol by Ag NPs, Why CTAB is not good as much as SDS with the same reaction? What is the main reason with...

24 July 2021 4,859 4 View

What is the successful procedure for synthesis core and shell quantum dots like CdSe/ZnS?

I have synthesized CdSe with green emission but the shell of ZnS couldn't formed. The procedure is cadmium oxide and Se dissolved in oliec acid with heating in the presence of paraffin liquid,...

15 September 2020 8,044 2 View

hello,in Cisco packet tracer where can I find the data storage from the sensors in the figure?

hello, where can I find the data storage from the sensors in the figure?

26 July 2020 1,849 0 View

Hot press machine curing time?

Dear All, I am planning to cure (T700GC/134) carbon fiber prepreg, using hot press machine. Could anyone please suggest the curing time, temperature and pressure for me? Your cooperation is much...

17 December 2019 5,926 1 View

Are the frogs reproduce by parthenogenesis?

Reproduction by Parthenogenesis

08 October 2019 8,487 5 View

How to correct the Rule of mixture?

The rule of mixture is not always correct, what are the main factors that should be added to the Rule of mixture to make it correct?

31 March 2019 4,715 2 View

Which machine learning classifier will be used in numerous representation of Arabic corpus datasets. whether the accuracy rate has increased ?

Which machine learning classifier will be used in numerous representation of Arabic corpus datasets. whether the accuracy rate has increased compared to previous research techniques. ?

05 February 2019 5,322 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

How to predict Shine-Dalgarno sequence ?

Hello, I am Master student under molecular biology. I want to find the Shine-Dalgarno sequence after performing a BLAST search in TargetRNA2. Could you please suggest a web tool to predict the...

07 July 2024 9,598 1 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Livro DNA Sequencing Protocols de Colin A Graham ?

Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.

11 June 2024 4,042 0 View