How do I design primer set for single mutation?

I have coordinates for my genes of interest and I want to design primer set to identify single mutation. I got the DNA sequences using UCSC browser and designed my primers using Primer3, however,...

15 December 2019 9,629 3 View

Issue with protein overexpression?

Hi, I am using a lentiviral vector to over express a protein in AML cells. I have cloned the flag-gene into the EIFalfa-MCS-SV40 vector. When I use this vector to simply transfect 293 cells, I can...

06 October 2016 903 3 View

ChIP-qPCR: same volume or same DNA concentration?

21 September 2016 4,154 4 View

Chip-seq. IgG and IPs have the same DNA concentration?

Hello, I'm doing a Chip-seq experiment and I've noticed that after DNA extraction I have the same amount of DNA in my negative control (IgG) and in the IPs? Do you think it's still worth it to...

27 February 2016 8,127 6 View

Is there a scale (could be a short scale or some items) for evaluating the perceived naturalness of scenes (e.g. landscape, neighbourhoods, gardens)?

this is for a pretest of some fotographs of houses (with and without plants nearby) and gardens (with more or less plants and other natural elements). we want to find out which of the stimuli are...

26 February 2015 688 5 View

Is there a scale to measure fast life history strategy (r-strategy)?

There are some measures for slow LHS (alhb, mini-k, hkss), but I could not find a scale for measuring fast life history strategy in humans. Does such a scale exist? Will it make sense to develop...

21 November 2014 2,827 11 View

Talking of preformed mediators, how about IL-1ra, IL-6, IL-18, MCP-1, and MIP-1beta?

I have been looking for cite worthy papers on the subject, but they seem to be hard to find. Thanks!

17 December 2013 1,507 3 View

Lymhpoma cell culture issues, help?

Hi! I need help for some issues in growing mantle cell lymphoma cell lines (REC-1, MAVER-1, Granta-519, Mino, Jeko-1). I noticed that it is a little bit hard to maintain a stable cell culture:...

09 February 2021 7,402 11 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Do family demographic processes link socioeconomic status to men's physical health?

Previous literature suggests that lower socioeconomic status (SES) men through higher family sociodemographic pressure and lower capacity of stress control, compared to higher SES men, are more...

08 October 2020 2,740 1 View

Which NCD is likely to increase the most after Covid-19 pandemic is over?

We have seen that the mortality among the Covid patients suffering from NCDs is very high. So we need to protect NCDs patients from covid infection.But a rise is expected among NCDs after the...

23 September 2020 4,080 4 View

Which strategies and tools you will recommend for the Hotel Industry to overcome the recession during and post-COVID-19?

During and post Covid-19 health crisis, the Tourism, Travelling and Hospitality industry is the one which is suffering more impact. I am interested in covering how the Hotel industry in particular...

12 May 2020 707 3 View

How sure are you, that after TUR-B for NMIBC, to have enough muscle tissue for correct histopathological examination?

Deep resection for correct histopathological analysis in TUR-B can be challenging for the surgeon with a low to mid-experience level. This leads to inadequate analysis, possibly postponing radical...

11 April 2020 5,713 3 View

Does Human body develop resistance or anti bodies against COVID 2019?

Does Human body develop resistance or anti bodies against COVID-19! Once a patient is fully recovered from the said disease.

24 March 2020 9,502 23 View

How do I transfer documents relating to case study to be reopened for the requested enquiries?

MT-pyramid Wholistic Geometric Quester: mtpyramid.weebly.com has explorations on diagnoses and treatment; The case suffered long period of repeated "mistakes"; Transformed to Wholistic Geometric...

15 March 2020 3,395 3 View

What precautions should I take working with Oleic Acid?

I'm working with UpConversionNanoParticles NaYF4:Yb,Tm using Octadecen as solvent and Oleic Acid as surfactant, I have read that this kind of UCNP are photostables but I dont know if the OA can...

01 February 2020 2,739 1 View

Looking for the cause of death in myocardial infarcted mice?

10 November 2019 527 3 View