Can someone share the methodology for syncytial assay?

More Sreenivasa Bp's questions See All

Will pacI undigested recombinant pAdEasy DNA interfere in transfection?

~16ug of Qiagen plasmid mini kit purified recombinant pAdEasy DNA was digested by pacI in 20ul final volume. We used this one (@4ug/well in a 6-well plate) for transfection into 293 cells using...

31 December 2013 6,739 0 View

Which Fetal Bovine Serum (FBS) do you recommend for HEK293 cells?

I request only those researchers who are familiar with culturing of HEK 293 cells to answer this question. In your experience, which FBS is best suited for trouble free culturing of HEK 293 cells?...

11 December 2013 4,094 14 View

Can P-60 be called low passage for HEK-293 cells?

Recently we purchased HEK-293 cell from ECACC. It is supplied at Passage 60. But HEK-293 is available at a passage say 30 elsewhere (e.g., Microbix, Canada). I am just wondering whether we have...

06 July 2013 2,195 4 View

How to get rid of proteins less than 100KDa from a virus preparation? Need a small scale method for this purpose.

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04 May 2013 1,130 16 View

Working with Clone Manager for blunt end cloning

Hello, I am learning to use Clone Manager 9.0 for simulation of cloning. I need your help to understand how to simulate cloning of a blunt end PCR product into pCR-Blunt II-TOPO vector. I am...

05 June 2011 9,766 2 View

Protein size discrepancy of His-tagged protein

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01 February 2010 6,901 8 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

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Which is more important: The human factor or the technological factor in combating climate change?

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02 August 2024 6,773 12 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

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01 August 2024 4,606 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

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31 July 2024 8,141 13 View

Posthoc test lettering in JAMOVI?

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31 July 2024 3,333 4 View

How to make Boehm titration ?

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29 July 2024 4,048 4 View

How to retain the a GFP tagged gene expression in stable cell line?

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29 July 2024 5,007 0 View

Recovery Viurses from bacteria genome?

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In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

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Peter C Charles

Check out the articles posted by S. R. S. Rangan:

https://www.researchgate.net/publication/18816521_Syncytial_giant_cell_focus_assay_for_viruses_derived_from_feline_leukemia_and_a_simian_sarcoma

Article Syncytial Giant Cell Focus Assay for Viruses Derived from Fe...

Guenther Keil

may be These references help:

1. Viral glycoprotein-mediated cell fusion assays using vaccinia virus vectors.

Bossart KN, Broder CC. Methods Mol Biol. 2004;269:309-32. Review.

2. A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors.

Edinger AL, Doms RW.

Methods Mol Med. 1999;17:41-9. doi: 10.1385/0-89603-369-4:41.

Good luck,

Guenther