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Questions related to Vectorization
I have my gene synthesised and cloned into pGenDONR by Genscript = 6584 bp. I transformed E coli TOP10 and miniprepped the plasmid for propagation and storage. On an agarose gel, the...
24 November 2021 2,798 8 View
Hello, I recorded a 2D HN spectrum on Bruker TopSpin 4.1.3 on a 800 MHz spectrometer and when changing the referencing I accidentally set the Spectrometer Frequency SF to 0 Hz in the nitrogen...
24 November 2021 9,432 2 View
Suppose I have created the matrix C=[1 2 3; 3 4 5;6 7 8;9 10 11;12 13 14;15 16 17]; and I would like to pick up any row vector randomly from this C matrix. Which MATLAB command/function useful for...
22 November 2021 7,273 8 View
I need help. I want to construct a single vector with 10 genes about 14 KB for overexpression in Chlamydomonas reinhardtii. which strategies is best to construct this vector. is there any solution?
20 November 2021 5,683 1 View
Hi, I am making a 3rd generation LV expressing my gene of interest. I know that putting a polyA signal within the LV genome will lower the vector titer, but what happens if I put it in the...
17 November 2021 7,028 1 View
Hello! I was wondering if anyone can provide me with an insight on how an exponentially decaying field is caused due to having an imaginary wave vector? regards Ketan
15 November 2021 8,325 11 View
I need to clone my small size insert of 33bp only (in vitro anneled duplex oligo) into Psicheck2 plasmid (6.7 KB) by single digestion with NheI, but my ligation is getting failed every time. I was...
08 November 2021 3,494 10 View
Hello, I have a mutation in cystathionine beta synthase (CBS) gene cloned in bacterial pET28 vector and I would like to clone it in a yeast vector. I already amplified the gene by PCR, did PCR...
06 November 2021 3,279 1 View
Hi! We are in need of multisite cloning entry vectors. Specifically, pDONR221(P1-P4) and pDONR221(P3-P2). Does someone in Europe have some to send us? I would be ever grateful!! All the best
01 November 2021 912 2 View
When I tried building the peptide sequence "KEPAPTT" on Pymol, I get the following error after trying to add alanine:...
31 October 2021 5,140 3 View
Hello, I am looking for a replicative vector to transform Lactobacillus species. Can anybody help me? Thanks! Rocco
27 October 2021 9,724 1 View
Hi everyone! I'm trying to insert a ssDNA(~100bp) to my vector(~10000bp) by Gibson assembly. In my plan, the vector are digested by BamHI and NdeI overnight, gel purified and then incubated with...
26 October 2021 2,279 5 View
Hi Can anyone share Pichia pastoris pGAPZalpha A plasmid with me. Thanks in advance
21 October 2021 1,369 1 View
Hi everyone, I am just trying to do Gibson assembly using HiFi DNA Assembly Master Mix with 4 inserts and a double digested vector with BsaI and BbsI. The information about inserts is as below: 1....
19 October 2021 1,843 1 View
Hi dose any one have any experience in BioBrick cloning? new in the field and trying to find a registry of "AVAILABLE VECTORS", I am looking for vectors with Chloramphenicol resistance to be...
13 October 2021 7,057 1 View
I am analyzing the influence of geography and river basin on gene-flow of an endemic frog. I am interested in performing dbRDA. I am using an individual based genetic matrix. I understand that we...
12 October 2021 7,848 1 View
Is there any application for fractional vector calculus (FVC) in electro-statics? Or where the FVC appears in EM theory?
08 October 2021 9,444 1 View
I have a design which will cut the prokaryotic vector about 30bp away from the ampR promoter. Will this cause problems or should I look for one further away?
04 October 2021 4,098 1 View
I have an objective function which requires a floating-point number as input and I want to maximize value of that objective function. Now my initial population in DE is like this. Population Size...
27 September 2021 7,614 3 View
Experts say knowing the vectors present is a key factor in putting an end to high malaria prevalence. Nigeria accounts for the highest malaria cases and global malaria death . .. Finding the way...
19 September 2021 7,347 8 View
Hi, I am trying a cloning over a long time. My vector is of about 4.7 kb and insert 1.4 kb. I am using Xho1 and Kpn1 as restrictions. I am attaching some files for details. In the first image I...
05 September 2021 3,601 5 View
I am trying to clone/insert a second gene to the pRSFDuet1 vector but the second gene is not showing up in the gel after cloning. What precautions should be taken to successfully clone the second...
05 September 2021 8,279 1 View
Hello. I recently cloned some plasmids for shRNA. I ordered PAGE-purified shRNA duplex oligo, and inserted it in lentivirus vector for shRNA(addgene cat.no. 8403, pLKO.1-puro) using AgeI and MluI...
03 September 2021 4,970 10 View
Hello dear friends I am working on gene cloning. One of the stages is pCAMBIA 1304 vector extraction. The low concentration of vector at the final stage of pCAMBIA 1304 vector extraction, is my...
31 August 2021 2,861 3 View