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Questions related to Vectorization
hi, I'm new to cell cloning Technics. In my case, 4t1 cell-lines was inserted IRES-vectors with one specific gene and also empty gene (for control). As I sub cultured, I found more green...
11 December 2020 9,040 3 View
hello! I have a pAR 3038 vector and would like to gather more info about it (map, databases that refer to it etc.) I do have this info: Vectors for expression of cloned DNAs by T7 RNA polymerase...
09 December 2020 2,414 2 View
I am trying to knockout gene at a specific location using CRISPR/Cas9 system but the issue is my spacer gRNA (20nt) contains 4Ts(TTTT) which signals as poly III terminator. If you have any idea...
07 December 2020 715 5 View
To analyze gene-miRNA direct interaction, I want to perform a luciferase reporter assay. I plan to clone the 3’UTR of interest in pGL3 basic vector ( which is a promoter-less vector). Can I use...
24 November 2020 2,911 2 View
While working with an SPSS file which produces a Spearman analysis on a pair of column I observed that the Value of N in the output is not the same. How is that possible? If I have 2 vectors X and...
13 November 2020 8,537 3 View
Hi everybody, I have a non linear system of 9 equations: F=A(F)F+B(F)g(F) F is a 9x1 vector of the unknowns, A(F) and B(F) are 9x9 matrixes which depend on F ang g(F) is a 9x1 vector which...
13 November 2020 10,089 6 View
I want to generate some nice prediction plots from my MRQAP model. I've laid out my process below, and would be very grateful to get anyone's insight, as I'm not seeing much written about this...
06 November 2020 1,412 2 View
Hello everyone, I have .car, .mdf file generated using Material Studio 18.1. To get the LAMMPS data file in PCFF format, I used msi2lmp tool available in LAMMPS package. But when I did...
29 October 2020 4,998 2 View
Hello all, I get a problem that needs all you support. The question is that I need to transform a set of points on an arbitrary plane to 2-D dimensions, Oxy. Also, the distance between points...
23 October 2020 9,494 4 View
I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...
20 October 2020 3,036 3 View
i went through many literature to find out the exact viral vector but it was generally mentioned m13 vector in the papers not specific about the name, can anyone suggest me which m13 vector can...
19 October 2020 2,764 1 View
Usually several methods are used to calculate uncertainty. At the time of analytical method development and validation for pesticide residue determination, it is necessary to estimate uncertainty....
19 October 2020 8,726 10 View
I am trying to clone my insert into a specific vector. I am using double digestion protocol with NEB restriction enzymes. I am getting self ligation of vector so ligation of insert is being...
14 October 2020 5,773 3 View
I was reading a paper that used escherichia coli origami DE3 strains to express a certain protein and the vector used was a pTrcHis-Topo with a trc promoter. I am wondering if they made use of the...
13 October 2020 2,629 3 View
Recently I am working on ArcGIS and faced some problems like when I covert a classified raster data to vector using Raster to Polygon in Arcgis, the pixels near the boundary are missing and that's...
09 October 2020 709 4 View
I am not sure; is AAV2 best for SY5Y and HT22? Is there any comparative study for these lines? Also, Can i use same serotype vector for the two cell lines?
03 October 2020 3,417 2 View
Hello I have a system of linear equations composed of 11 equations and 11 variables, I solved it through Excel and MATLAB in the form of AX=B. While the matrix A , matrix B and the inverse of...
03 October 2020 10,010 7 View
I'm now using Gene Pulser Xcell (Biorad) to transform a vector (pUC118) into E.coli DH5α. In fact, I did this procedure many times and got satisfying rssults. However, I encountered a problem...
03 October 2020 8,775 1 View
I want to use ORI in my plasmid vector for replicating it in SH-SY5Y cells like in 293E or 293T cells with SV40? Is there any ORI that can be replicated in SY5Y cells?
25 September 2020 2,646 3 View
Can please someone help me in calculating and plotting the RMSE of MUSIC versus SNR of the attached matlab code
21 September 2020 4,698 0 View
I have implemented Switching table based direct torque control of three phase induction motor. But now i want to implement virtual space vector based direct torque control and for that purpose i...
20 September 2020 8,537 1 View
I have been using Gibson Assembly to insert a 7kB fragment into an 8kB linearized vector. After assembly many colonies form, but colony PCR on the transformation consistently shows a 1.5 kB...
19 September 2020 441 3 View
Hi, I have some trouble with my HEK293-hTRPV1 stable cell line. I made a 293-hTRPV1 stable cell line with Flp system from Thermofischer...
17 September 2020 5,354 2 View
Hello I recently transformed bacteria with my vector and insert ligated construct. In order to check the presence of both the vector and insert, I mini prepped 20 bacterial colonies and did...
15 September 2020 7,596 4 View