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Questions related to Vectorization
Two protein were docked by H-Dock server, now we want visualize the interaction such as H bond between them. As you know PDBsum server can do this, there is any link to do it?
30 August 2022 8,569 5 View
Please give answer with references.
22 August 2022 6,048 0 View
I would like to assess transcriptome upregulation or down regulation in my plants between treated and control. I know there is the TruSeq RNA Sample Prep Kit v2 - Set A for easy mRNA isolation and...
03 August 2022 6,945 2 View
What happen If rotor of induction have only rotor bars and without laminated iron core ?
02 August 2022 1,698 2 View
Hi everyone, I have been trying to figure out a reliable way regarding how to present % intergroup difference of a marker level between study and control groups in a clinical trial and I am...
02 August 2022 6,176 2 View
I am researching into church governance and management. What theories can I adopt?
23 July 2022 8,414 3 View
Hi all, I'm currently working on DNA-Stable Isotope Probing and I'm trying out a protocol for Isopycnic centrifugation for DNA fractionation. Most of the protocols that I'm consulting all used...
21 July 2022 6,219 0 View
We have a server installed in our department with the following configuration. Dell PowerEdge R740 2 x Intel Xeon Gold 6128 12/24 cores/threads Core frequency: 3.40 GHz 256 GB RAM SSD RAID1 480GB...
29 June 2022 5,119 1 View
Looking for the calculation or methods to determine the inertia, J (kgm^2) and friction factor, B (Nms) for 3phase induction motor.. Thank you!
11 June 2022 8,673 0 View
Dear i would like to calculate the absorption spectra for different orientation of circular polarization of the pumb laser for 2d materials . to do this calculation i can use turbo_lanczos.x then...
10 June 2022 5,171 1 View
I use the bitcoin price and other block chains data to do the Johansen Cointegration test. The result show that they have two Cointegration. Then, I construct the VECM, but I don't know how to...
07 June 2022 4,578 3 View
Does anyone know if it is possible to use this LSK109 sequencing kit (Oxford Nanopore) to sequence single stranded cDNA?
29 May 2022 3,196 0 View
Hi everybody, I purified a recombinant Pathogenesis Related protein belonging to class 10 (PR-10) and I want to test if the enzyme has RNase activity. I read that is possible to test that activity...
13 May 2022 3,711 2 View
I'm currently performing Agrobacterium transformations of immature wheat embryos for a CRISPR-Cas9 project. I do not have any colleagues with experience of working with wheat transformations and I...
26 April 2022 1,351 3 View
We know that there's T equivalent circuit and inverse Γ-equivalent circuit for 3 phase induction motor(IM). When the IM is operating under normal situation, the windings are symmetrical, so all 3...
13 April 2022 8,223 4 View
I want to know how can I calculate absorption by poynting vector and electric field and I need integrate for that.
03 April 2022 6,170 0 View
I have a genomic DNA library digested with BamHI enzyme and ligated into a pUC19 vector. I am planning to amplify specific genes. Is it necessary to first add BamHI enzymes to release the genes...
21 March 2022 9,305 3 View
Does pcDNA3.1 vector not work for HEK293T cells?
18 March 2022 3,754 2 View
Hi Dear all, I want to calculate the normal vectors of the Tyrosine amino acid ring and the chromophore rings. In that way, I want to calculate the angle between these vectors and distances. I...
16 March 2022 5,834 1 View
To diagonalize a covariance matrix 500+x500+ is extremely difficult, almost impossible. To maximize a mean squared projection of the vectors on some direction a=(a1,a2,.....a500+) You are to solve...
12 March 2022 4,080 4 View
Hi, Is it possible to add 2 of the same genes under the same promoter to an integrative vector? My goal is gene upregulation so I am adding a strong promoter to the gene and want to add 2 copies...
09 March 2022 6,439 3 View
- I’m looking at evaluating level of agreement b/w 2 scores taken from the same person, 2 wks apart - I have produced a bland-Altman plot. Mean differences b/w scores normally distributed - as...
09 March 2022 6,900 0 View
This is our first time reprogramming iPSCs. We reprogrammed fibroblasts using episomal vectors. Attached is a picture of reprogrammed fibroblasts at 4x on Day 19 (15 days of N2B27 +FGF and 4 days...
07 March 2022 2,417 5 View
I am inserting an shRNA loop into the pLKO.1 vector. The shRNA will be under the control of the U6 transcriptional promoter in this vector. From what I understand the transcriptional start site...
03 March 2022 3,097 2 View