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Questions related to Vectorization
In the lab we used to use Matrigel (10 µl) for coating of 8-well Ibidi chambers and added cells with DMEM (Gibco 11965092) + 5% Matrigel. This worked fine but if we wanted to do imaging you needed...
02 March 2022 1,026 0 View
Hello everyone, I'm currerntly working with a Cas12a vector, which contains a cutting site for Eco31i and a 19 bp direct repeat, which is necessary for the Cas12a to function. After the...
22 February 2022 3,229 1 View
Hi, I have tried to verify the function of one enzyme while my target protein is insoluble in E.coli expression system , I have two thoughts: 1. trying the yeast expression system 2. try to find...
22 February 2022 3,231 3 View
Hi scientific community, I have defrosted (from -20ºC) one bottle of cRPMI (complete RPMI media) then I have prepared all the aliquots (10 mL) under sterile conditions and all of them have been...
15 February 2022 5,723 0 View
I'm recently making research on induction motor parameter identification and found a research matching my purpose, but I don't know how to build this "ABCabc model of an induction motor", which is...
10 February 2022 6,579 5 View
I would like to know the specific reason why the angle with respect to the positive direction of the x-axis is used when calculating for the components of a vector.
07 February 2022 10,060 2 View
I have two complex vectors, let's say a and b. How to compute cosine similarity between a and b using MATLAB?
26 January 2022 2,349 4 View
thanks
23 January 2022 625 3 View
So I have been trying to construct a phylogenetic tree based on a collection of ITS sequences of a library of fungal isolates I cultured. However, after constructing it on MEGAX with my aligned...
22 January 2022 5,942 3 View
Kindly explain the mechanism behind it, I shall be very thankful to you
21 January 2022 2,918 0 View
Hi! I am characterising the distribution of cells and their repartition on a structure in confocal images. I have measured the orientation and distance of the cells from the center point of the...
11 January 2022 1,198 1 View
I’m looking for some help troubleshooting my cloning process. I’m trying to ligate a shRNA into a plasmid (pWALIUM20, ~10Kbp) but have been unsuccessful many times. I’m using a double digest...
11 January 2022 6,419 2 View
Looking to use Cas9 for research in a startup (possibly lentiviral), and would like input on what are the legal requirements and how to obtain Cas9 expression vectors (Cas13 is available from...
09 January 2022 6,458 3 View
HI. if I have 7 industry dummy variables and another dummy for listing status all being dichotomous in my model. it results multicollineartiy issues when including all the dummies in the...
04 January 2022 2,755 1 View
When I inversion the component of magnetic vector , My、Mz shows a fit result , but Mx often shows positive and negative associated results or tiny value. So when I try to make a profile about...
13 December 2021 9,479 1 View
I'm doing a subcloning experiment with a pTNT vector and I treated it and my inserts (engineered with RE recognition sites) with EcoRI and KpnI, then incubated the digested vector with alkaline...
10 December 2021 3,813 3 View
Hi great Researchers, We are planning for animal trial where we will implant a PDMS based medical device for 2-3 weeks. I was wondering, can I use normal lab Oven (...
08 December 2021 6,002 3 View
hello to all.. I want to calculate the sum of the x component of velocity, the y component of velocity, and then the magnitude of velocity ... which commands can I use...
07 December 2021 4,034 1 View
I have estimated a VECM model in EViews and using Johansen test I obtained that there were cointegration vectors. The output for short-run equation of VECOM contains two cointegration vectors, I...
06 December 2021 8,659 8 View
This is a fundamental question about basic phage library construction. I know that people can construct library by either phage vector (eg. M13KE) or phagemid+helperphage. Based on my...
04 December 2021 8,709 4 View
I'm wondering whether it's possible to do a Kruskal-Wallis test on difference scores, since I can't seem to find an example of this online. I think it would make sense conceptually, but I would...
02 December 2021 2,187 5 View
I would like to ask a question. I have performed many measurements on my samples with the free space method using the vector analyzer: N5230A PNA-L Network Analyzer, 2-ports Now I would like to...
01 December 2021 7,330 13 View
I am working on BEM. I can compute its stiffness matrix A. (AX=B). X= surface potentials and their normal derivatives B= source vector I want to compute leadfield matrix and I don't know how I...
01 December 2021 9,526 3 View
Hi All, I have a protein sequence cloned in pET21+ vector. Below is the part of the sequence starting from T7 promoter to the ATG (of my...
30 November 2021 5,002 3 View