Vectorization

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Questions related to Vectorization

Does the quantum vacuum have analogies in mathematics?

Take, for example, such a concept as a minimum flow, that is, a gradient vector field, the level surfaces of which are the minimum surfaces. Then the globally minimal flow, evolving to an...

14 September 2020 8,704 13 View

Does anyone have a good source for designing cpf1/cas12a crRNA arrays?

Hello, I'm trying to design an array of 4 crRNAs for use with cpf1/cas12a both the As and Lb versions. I've found the scaffold and PAM sequence from digging through a few papers, but I can't find...

13 September 2020 4,106 2 View

How do you convert CADNANO vector drawings into .JPEG AND TIFF IMAGE FORMAT?

Hello, I am working on a DNA nanostructure but I am new to CADNANO. I need some help on how to convert CADNANO drawings into images like JPEG and TIFF. At the moment, my .svg output appears cut...

12 September 2020 4,669 2 View

How to locate stabile/unstable points of a bilinear dynamical system?

Dear All, I was wondering if somebody could direct me to a relevant result. We would like to understand asymptotic stability of the following system $$ \partial_t e_{i} = B_{ij} e_{j} +...

12 September 2020 1,347 1 View

Is it possible (panel or time series) cointegration including one lagged variable?

Dear all, I am certain that one of the variables in my vector can affect the rest only if it is lagged. For example, lets imagine an agriculture product which is planted in a year and harvest in...

11 September 2020 1,165 4 View

Can I ligate a blunt end of insert with sticky end of a vector ?

I am trying to ligate an insert that has Sca1 (blunt) and Xho1 (sticky) as 2 flanks with a vector that is cut with Sal1 (sticky) and sma1 (blunt). Does these ligate considering the sticky-blunt...

10 September 2020 827 4 View

In the cloning process during ligation how to resolve high-ordered combinations of plasmid vector or restriction fragment?

DNA ligase only recognizes and acts on the ends of linear DNA molecules, usually result in a complex mixture of DNA molecules with randomly joined ends. The desired products (vector DNA covalently...

09 September 2020 494 2 View

What can I do to convert the blunt ends of the gene to cohesive ends?

To construct a genomic library the vector is treated with a restriction enzyme which has yielded cohesive ends. But, the gene of interest has blunt ends. Please suggest a method to convert the...

06 September 2020 3,521 3 View

How to summary the result of comparison two means vector?

In multivariate analysis about the comparison two means vector, If the result of testing hypothesis By T square Hotelling is reject H0 that means mu1≠mu2. But when using simultaneous confidence...

02 September 2020 6,443 3 View

How to create a vector with a specific sequence of species to be extinct in the “external” method of the bipartite R-package?

Dear peers, I am working with extinction models in interaction networks using the bipartite R-package. I can determine the order in which species will be removed based on abundance and degree or...

31 August 2020 2,412 2 View

Has anyone done magnetic target localization using Genetic Algorithm?

I am trying to model magnetic target localization in MATLAB using those algorithms. So essentially what I want to output is the position vector of the target (x, y, z) and the magnetic moment...

29 August 2020 9,943 2 View

Can anyone please check why this code is giving me errors??????????

library ieee; use ieee.std_logic_1164.all; entity group_c is port(red : out std_logic_vector(15 downto 0); green : out std_logic_vector(7 downto 0); RL :in std_logic_vector(1 downto...

25 August 2020 8,591 3 View

Hi all, can OLS be used on single equation when it has two (2) cointegrating vectors?

Let suppose an equation Yt = a + b1Xt + b2Zt + b3Ft + e where Y is the dependent variable and X, Z and F are independent variable. If johansan cointegration shows 2 cointegrating vectors then...

24 August 2020 3,734 12 View

How can i obtain electric displacement field in Piezoelectric bonded joint using Abaqus?

Mathematically, electric displacement is a vector quantity defined as {D}=([e]{E}+{P}), where {E} is local electric field, {P} is Polarization per unit volume and [e] is the third order tensor...

21 August 2020 4,875 1 View

What sequence to use for overexpression of VEGF-A isoform with 165 amino acids?

Hi, I am working on a project where I need to overexpress VEGF-A isoform 165 aa in NIH 3T3 cells and was planning on using lentivirus with the vector backbone pLVX. I found that the precursor...

19 August 2020 476 1 View

Spatial panel vector auto-regressive (VAR) model OR Spatial panel vector error correction model codes (VECM) in stata?

I am looking for codes/Package available for Spatial panel VAR model or Spatial panel VECM model in stata.

18 August 2020 1,764 9 View

Is their any standardized protocol Construction of Corynebacterium glutamicum vectors for producing glutamic acid?

Corynebacterium glutamicum is an important industrial strain used for the production of amino acids and vitamins. So I want to know is there any standardized protocol for the Construction of...

14 August 2020 1,626 1 View

Should I use equimolar amount of inserts for gibsson to work?

Hello, I am trying to assemble 2 fragments into a vector. The first fragment size is 820 bps, second fragment is 141 bps and the vector is 5373 bps and all fragments and the vector have 25-30 bps...

09 August 2020 6,976 0 View

Will low 260/230 (nanodrop) after cleanup affect ligation?

I digested and dephosphorylated a vector, and digested a PCR amplicon (my insert). Since I had to use a column to cleanup my vector digest before ligation (since CIP is not fully...

08 August 2020 3,821 1 View

What is the fundamental difference between KSOM and LVQ models?

The Kohonen self organising maps(KSOM) and the Learning vector quantization(LVQ) both classify patterns. It is said that both these two being that the topological structure at the output unit is...

05 August 2020 7,174 2 View

Why my sequence for the 9 samples were so bad?

I had subcloning my three genes in pNUS-HcN vector using NEBuilder kit and I got conformation using restriction enzymes which I used them to cut the vector and also I confirmed that using PCR...

30 July 2020 4,350 1 View

Advice on Gibson assembly reactions using NEB kit?

We are new to Gibson assembly and are using the NEB Gibson assembly master mix. Reactions with vector alone (no insert) give no colonies. Adding an inset of 660bp gave 10s of colonies. But a large...

24 July 2020 6,263 2 View

How to ligate small insert to big vector?

I would be so grateful to everyone who considers this problem. I want to ligate about 120 bp insert DNA into a vector with 8 kbp size, and I have tried the molar ratio of insert/vector 7:1, 10:1,...

21 July 2020 1,920 3 View

I am planning to express my protein in Pichia and would like recommendations on the best expression kits or individual components for expression?

I have no prior experience with expression in Pichia. I would like recommendations on individual Pichia cells and vectors, or expression kits. I also want to make decisions about secreted /...

20 July 2020 1,354 1 View

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