07 July 2023 0 7K Report

Please share the protocols for transformation in diploid strain of fission yeast

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I want to remove Urea from my protein sample?

I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...

06 August 2024 1,350 4 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to draw photon-magnon coupling color plot in origin?

Please help me in plotting the photon-magnon coupling plot as shown below

14 July 2024 1,632 1 View

Should the final concentration of DMSO in the wells of the plate be the same for different concentrations of compounds - studies on cells?

Hi, My research will consist in examining the effect of compounds at concentrations of 10(-4)M, 10(-5)M, 10(-6)M, 10(-7)M, 10(-8)M on cells. I prepared 100 mM stocks of compounds in 100% DMSO....

21 April 2024 3,032 0 View

What software do I use for brine wastewater nutrient and mineral, biological statistical data analysis and how do I use them?

Good day Water, marine Biologist scientist, Environmental Engineers,and Industrial Ingineers, chemists and chemical Engineers experts I hope this message finds you well. I am currently serving as...

06 April 2024 3,681 4 View

What is the difference between optical bandgap and electrical bandgap?

how we can interpret it??

18 March 2024 4,915 2 View

What is the amount of radiation in space?

amount of radiation in space

09 March 2024 3,855 0 View

Error reading k point # 1, what parameter I have to change?

I have done ph.x, q2r.x, matdyn.x and have got the output. But, while running plotband.x I am getting error like "error reading k point # 1"

03 March 2024 8,899 0 View

How many atomic bomb surviours of Hiroshima and Nagashaki are still alive ?

Atomic bomb surviours of (out of ~ 105500 or so reported) of Hiroshima and Nagashaki in Japan

02 March 2024 8,583 1 View

What should be the characteristics of Matrix in MALDI -MS to produce (M-H)- ion in negative ion mode?

MALDI( Matrix Assisted Laser desorption Ionization) Matrix use in MALDI in positive mode and negative mode

18 February 2024 5,919 1 View

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Bimodal Distribution, Split or Transform for ANOVA?

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My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

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Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

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I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

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