Does anyone know if crispr/cas12 detects cDNA?
This question is in relation to the journal written by B.M. Kavuvalu and others entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya"....
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This question is derived from the journal article “The Transition from Gel Separatory Serum to Lithium Heparin Gel Tubes in the Clinical Laboratory,” written by Oguzhan Zengi. The journal...
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I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
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I have been working with HepG2 cells for some time with no problem. The past couple months when I've been plating into 96 well plate for experiments I am noticing the cells are not healthy or...
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I have done a number of MTT assay's for a study I am a part of, twice the results showed a very low viability and this crystal formation. I did a later assay following the same procedure, using...
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Hello! I really hope anyone can help me out. For my master thesis I am comparing cancer cell clones and their reaction to certain targeted inhibitors. Now I would like to perform a two way ANOVA...
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How has the concept of liberty changed in the era of Bid Data
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Dears, I need to buy bovine Photoreceptor Outer Segment. I asked for the 98740 product to InVision BioResources, but they do not reply to me. Do you have other suggestion? I checked in...
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In our thesis we did a reasearch about antioxidant acitivity of SOD. To get results we used the formula according to SOD kite Assay Cayman. We understood the formula the following way: the more...
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Blood donation intends to lower the blood pressure and risk for having heart attacks. But does doing blood donation from the donors' affect their immune system? If so, how does it do specifically?
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Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
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We aim to measure the RNA expression of p53 and K-RAS biomarkers in colon cancer samples. We extracted the RNA from Rneasy FFPE kit and converted the it to cDNA and did real-time PCR using Cyber...
08 July 2024 7,781 6 View
Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...
07 July 2024 9,206 4 View
500 ng RNA is converted to cDNA to be genetically expressed by qpcr sybergreen dye, 1 ul primer F, 1 ul primer R, 10 ul master mix, 1 ul cdna template, and instead of a complete total volume...
06 July 2024 5,478 4 View
I am doing qPCR but my HPRT1 Ct is not coming at the same point. I see a 2 cycle spread in Ct. I have checked my RNA quality A260/280, 230/280 values, did ethidium bromide gel. I used superscript...
04 July 2024 5,605 3 View
I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !
04 July 2024 3,068 3 View
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
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Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
We want to do qPCR from single cells extracted from patch-clamp recordings. As the RNA content of each cell will be very small and to prevent further degradation, i will not quantify it, instead...
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