Does anyone know if crispr/cas12 detects cDNA?
Hello, we have been trying to run MSTRAT Software for developing a core collection of Chilean Garlic genetic resources, but we keep getting the error: ether couldn't execute...
25 November 2020 9,748 2 View
Hello everyone, I am having troubles with the bubble point measurement to find the pore size of my self-fabricated microfiltration membrane, and I couldn't find a proper answer anywhere else. It...
16 November 2020 7,574 1 View
I can find very general maps showing the tidal extent in the Thames, but does saline water also travel up its tributaries? Can anyone send me or link me to a map showing the extent of estuarine...
29 October 2020 8,892 1 View
Hello all, I was wondering if someone could help me. I have read a lot of articles that mention using Betamethasone at clinically relevant levels in mice yet no one has mentioned the half-life. I...
08 September 2020 2,255 2 View
I am looking at the association of two molecules within a cell (expressed as a %association of one with the other). I have looked at this association at three different time points (i.e 6h, 12h,...
01 April 2020 510 4 View
Hello there, Before I begin my experiments, I would like to make sure all my mice in my experiment have proper vision and hearing. However the only test I know that would work is administering a...
19 February 2020 5,766 2 View
I've been studying plant metabolism and in my lab we ar used to measure superoxid dismutase activity using the methodology of Giannopolitis e Ries, (1977), using the nitrotetrazolium blue...
17 December 2019 7,272 1 View
I am interested in researching intermittent fasting (IF) and caloric restriction (CR) in relation to hypothesized positive effects on oxidative stress in the nervous system, specifically in the...
09 September 2019 7,012 3 View
I am trying to create a FIS fuzzy logic file on Matlab. I am referring myself to a pdf which has the rules and triangle form of the membership. The display range of the function is from - 0.2 to...
09 August 2019 8,016 6 View
Hi everyone. I tried concentrating and precipitating my protein using ProteoExtract Protein Precipitation kit. I did not have any of the buffers suggested to dissolve so I used RIPA buffer....
26 February 2019 3,141 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...
23 February 2021 809 3 View
For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...
23 February 2021 7,051 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...
17 February 2021 7,802 2 View
Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...
14 February 2021 3,129 8 View
