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Questions related to Vectorization
When teaching future physicians we propose them problems to solve. A popular kind of problems is devoted to precipitation of glasses or contact lenses for shortsighted eyes because myopia is...
22 June 2023 3,446 4 View
A software design is a plan or a blueprint for building a software program. It is a high-level representation of the structure, behavior, and functionality of the software that guides the coding...
19 June 2023 6,526 6 View
Thank you.
18 June 2023 5,121 0 View
https://www.surveymonkey.com/r/5KQP3D6 For all the experts in green neighbourhoods certification systems, urban planning, or sustainable development I'm currently conducting an expert-based AHP...
15 June 2023 9,731 2 View
It's easy to suggest PHYSICAL STIMULATION techniques but what is the point if no one can name any EROTIC TURN-ONS that cause female arousal?
14 June 2023 392 0 View
I am trying to transfect an sh-rna in my mammalian cells using lipofectamine 2000 reagent but every time transfection does not completely. Im using 1.5 ug of dna 5ul of lipofectamine and 200ul of...
13 June 2023 8,621 0 View
Hello everyone, I have a recombinant plasmid always getting overlapped peaks when sequencing. The sequencing primer is located on the backbone of the vector, tens of nts ahead the cloning site,...
08 June 2023 8,633 2 View
Dear community, Does anyone has experiences with bacterial cell free system? if any bacterial promoter can exhibit strong strength as high as T7 promoter, or quite similar? Thanks!
02 June 2023 1,703 0 View
after adding Isopropanol, and centrifuging, I’m not seeing visible white pellet at the bottom of the EP tube, is that means I proceed wrong ? please need some help !
25 May 2023 1,528 3 View
Can anyone explain the change in properties in semiconducting or conducting nanomaterials using the free electron model?
24 May 2023 7,074 0 View
What do we expect of a sex researcher or sexologist? Surely they should be able to differentiate between erotic fiction and reality?
24 May 2023 3,387 2 View
Im working on avian leukosis virus subject. therefore I want to collect some data about "updates on immunity and diagnostic tools" thanks for your attention and help.
22 May 2023 9,201 0 View
I am looking for one but I could not get an info. I only found SmartPLS for such a tool, but I am looking for a free alternative.
22 May 2023 4,927 4 View
We are comparing different DNA extraction methods and we use qPCR for analysis. We conducted PCR on 2 amplificators, but they had pretty different results. For example, one of the methods had 30...
20 May 2023 6,635 1 View
primers for detection of mutation
19 May 2023 2,637 2 View
Hello fellow researchers! We are trying to transform Arabidopsis with pCAMBIA2022, but we realized that the Chloramphenicol resistance inside the vector will not be compatible with the Agro...
19 May 2023 3,840 1 View
Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...
17 May 2023 873 3 View
/profile/Christian-Valentin contains 7 publications /profile/Christian-Valentin-4 contains 334 publications
07 May 2023 5,481 1 View
religious movements, mysticism,
05 May 2023 6,039 2 View
Which type of soil absorbs more water and temperature change chemical or mechanical weathering?
01 May 2023 9,030 2 View
I had performed ligation reaction at 4 °C (overnight) and 23 °C (4 hours) and when i transformed my ligation mixture using DH5-alpha competent cells, colonies appeared only with 4°C ligation...
26 April 2023 4,751 0 View
I'm trying to build a cosmid for B. subtilis, but there are no commercially available cosmids with a gram-positive ori already installed. I was thinking of obtaining a commercial cosmid (SuperCos...
20 April 2023 4,581 0 View
I am working with Xanthomonas. I want to inactivate or silence a gene that appears in the plasmids that are carried by these bacteria themselves. How can I do that? Any ideas? Thank you
20 April 2023 7,151 5 View
Having visited Krakow a few years ago and seeing St Adalbert's church in the market square, I am interested to know more about its age. When was it investigated and what was found out about the...
18 April 2023 5,231 4 View