Thanks for the answers.
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View
I use the same primer set and same conditions. The first PCR product from cDNA gave about 800 bp. Reamplification of the template gave 700 bp. A second amplification gave 600 bp. Interestingly, I...
24 February 2020 4,346 8 View
An insert length of 811bp (17ng/ul) and digested vector size of 5.6kb (35ng/ul). --100ng vector amount--. Am I right in using the following respective volumes of insert and vector? 2.5ul and...
06 May 2019 7,036 3 View
I'd like to be methodical in deciding which buffers to use per Western Blot Experiment. A thorough system of deciding which combination of buffers to use for each WB experiment would be helpful....
17 December 2017 5,382 1 View
I know in Arabidopsis people will often use something like tubulin, which is constitutively expressed, as something to normalize the amount of gene expression. I've seen people use 16s rRNA for...
13 March 2013 1,668 1 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
How to upload pdf of my articles published?
03 March 2021 2,183 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
There are some books published in LAP. Shall I add it to the RG?
02 March 2021 2,753 1 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
it is published by Juniper
28 February 2021 354 4 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...
28 February 2021 7,800 6 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
