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Questions related to Vectorization
When a vector field has a non zero curl, the curl might describe a magnetic field also implying the presence of an electric field. This question is about vector fields with curl of zero. Gradient...
22 June 2021 4,270 1 View
Hello! I am currently using pRSETA vector to clone some urease mutants by homologous recombination mutagenesis. But the sequencing results always show different undesirable single-base insertions...
22 June 2021 7,421 2 View
I use SMARTvector Inducible Lentiral shRNA vector, which include 2 promoters: costitutive promoter, which control the expression of puromycin resistence and TET-On 3G transactivator and inducible...
16 June 2021 3,109 1 View
I recently successfully made a plasmid using 5 parts (one of the parts was the vector backbone). I used the GeneArt Gibson Assembly® Cloning mix. Out of the 52 colonies that I screened (using...
15 June 2021 1,409 3 View
I would like to introduce the CBD as an affinity tag at the C-terminus of my construct. For that which CBD gene do I need to look at. So that I can purify my construct later on with Chitin beads....
13 June 2021 354 3 View
Hello, I am doing cloning using the pJET1.2 blunt vector (2.9 kb). The size of inserted gene is around 2.4 kb. When I transformed it into E. coli, I got some colonies and confirmed the positive...
09 June 2021 7,408 2 View
How I can design or calculate the aeration tank volume of the Extended aeration sludge process design? The COD = 1800, BOD 600, TSS 300 and TKN 200. If COD needs to reduce to 70. (Units are mg/L)....
06 June 2021 424 5 View
Gradient strength vs gradient amplitude. Is a 3T scanner with reduced gradient strength but higher amplitude as good?
02 June 2021 1,246 4 View
When it comes to spatial data, how can we calculate the uncertainty of raster data (e.g. temperature, elevation) and vector data (e.g. lake, forest, road)?
31 May 2021 3,603 4 View
Indeed, I work on fertilization and I would like to use this type of analysis. Many thanks in advance.
28 May 2021 7,430 4 View
pLAFR5 is a cosmid vector that has been in use for some time. If anybody has its sequence or knows where to get it, I would like to know. Thanks,
27 May 2021 730 2 View
Hi, I am traying to optimize the expression level of my protein cloned in pBAD vector. Currently, I am inducing it at 0.002 w/v but see a significant overexpression of my protein. The current...
27 May 2021 6,293 2 View
Usually it's the stereo microscope that we use to observe tick morphology; but my question is why the conventional light/optical microscope is not used for this purpose and if possible, is there a...
27 May 2021 9,253 4 View
Using TPS Series of Prof. James Rohlf for resolving problems related to morphology is a common technique in academia. I already used this series in previous successful works, however I do not...
27 May 2021 1,223 14 View
Vector analysis and vector calculus is part of the mathematical subjects that are typically taught to students at surveying and Geomatics at a BSc level. By looking deep to vector analysis,...
26 May 2021 1,466 1 View
Does anyone have recommendations for a good vector to make shRNA from 21 bp siRNA? A plasmid vector would be easiest for me, but I can also work with non-plasmid vectors.
18 May 2021 4,862 2 View
Say I have a none cubic Unit Cell with all its atomic positions defined fractionally (x, y, z defined as some fraction of the a, b, and c lattice vectors). Effectively, viewed in this way the...
17 May 2021 3,274 1 View
I want to get second opinion from experts for my priority vectors. Should i use a AHP-format survey (asking for pair-wise comparisons) or a simple questionnaire asking them to rank every criteria...
16 May 2021 4,294 2 View
It is well known that Fpg and Endonuclease IV can cleave AP site in double strand DNA. But can they cleave AP (Apurinic/apyrimidinic) site in single strand DNA?
13 May 2021 3,911 2 View
The direction of phasor determines whether polarization is left handed or right handed. For an observer viewing the wave approaching, the rotation of electric field vector in a stationary...
10 May 2021 6,906 2 View
I have an amino acid sequence of my target (KO) and there are different potential ORFs that could be initiated, as predicted by expasy-translate. Is there a way to check the strength of the Kozak...
04 May 2021 7,338 5 View
I'm trying to convert my vector into a Gateway destination vector so that I can insert a large section of DNA without relying on restriction enzymes. However, the only site available has a T3...
03 May 2021 3,958 1 View
I used the glutaraldehyde to cross-linked two different amino group compound to be my vector to transfected the cell. Here, I have a serious problem, I cannot resolve. After transfection, I...
30 April 2021 4,214 2 View
I have a small flask with ~100ml of Amp resistant bacteria in LB [Luria-Bertani] broth, that has been at 4 degrees tightly sealed for 26 days. I need to grow a larger culture of this same...
30 April 2021 1,455 5 View