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Questions related to Vectorization
This method for finding outliers when fitting data in a non-linear regression model is explained in the following article. Detecting Outliers When Fitting Data With Nonlinear Regressi......
27 March 2023 2,492 0 View
If we have 4 treatments for a trait and a SEM (pooled SE) for these treatments' mean, how to calculate SE for mean of each treatment from SEM?
19 March 2023 3,540 3 View
Is it okay If I collect the conditioned medium and centrifuge it and take the supernatant and storage it at -80 degree?
17 March 2023 4,073 0 View
I'm wanting to express 2 proteins (A,B) into a vector and transform cells with this. In the distributed construct strategy, each insert is cloned into a different, compatible vectors that can be...
12 March 2023 1,391 2 View
Hello, good evening. I have a question that arose when running an agarose gel for DNA. Some time ago, we transfected CHO cells with a plasmid and wanted to see if it could transcribe with that...
10 March 2023 9,719 4 View
I have succeeded recently to clone necessary elements for rAAV production into ONE universal plasmid. However, the size of this universal plasmid is 22 Kb. I am wondering if there is any...
07 March 2023 9,311 4 View
Is RMSD enough to access the stability of the complexes?
03 March 2023 8,834 2 View
I got the weird upside down 'U' shape amplification curve for my qPCR which gave me undetermined Cq value. My standards and samples are all diluted in TE buffer, the standard curve looks fine.
22 February 2023 5,771 0 View
Hello, I tried different methods of making refolding buffer including cystine, I dissolve it in HCL first but then when I raise the pH to 8 I can see it solidifying after raising the pH. how can I...
18 February 2023 2,527 2 View
Hi. In my correlation data, sample (A)'s correlation coefficient is r = 0.7160 and the p-value is p = 0.0458 (*) (n=8), when analyzed by Pearson's correlation test. On the other hand, sample...
16 February 2023 2,126 6 View
Dear greetings, What is the best method to analyze interview questions responses?
14 February 2023 910 5 View
Let A be an nxn matrix of integers and b be an nx1 vector of integers. Then, the problem: "Does there exist a vector of nonnegative integers x such that Ax=b?" is known to be NP-Complete. Assume...
11 February 2023 1,394 0 View
I am working right now on my Ph.D. project and as a part of it need to create an ScFv antibody. I have found a Vh Vl sequence, but it was created from an amino acid sequence from a 1995 article....
10 February 2023 3,531 0 View
I am searching for your tips & tricks for homogenous seeding of A549 cells in a 6 well plate. Do you have a protocol suggestion? Thank you!
07 February 2023 8,385 0 View
Please provide me the best formula for formulating silver paste so that I can avoid contact resistance when monitoring the performance of a supercapacitor.
07 February 2023 5,646 3 View
is there a specific ratio to follow during the addition?
29 January 2023 6,165 4 View
Hi everyone, I am facing an issue with running the denaturing Urea-PAGE to visualize the digested fragment of the Oligos (the first labeled oligos are Cy2, and the second one is Cy5). First...
19 January 2023 6,394 0 View
There are several global and freely available digital elevation models (e.g. SRTM, GDEM, ALOS...). However, they all have one thing in common and that is that these datasets are getting quite out...
16 January 2023 1,611 8 View
Are they non culturable/ lose their viability due to loss of their natural niche?
05 January 2023 4,981 3 View
Can you recommend peer-reviewed articles or papers which use the AHP survey to a set of experts? Your responses will be highly appreciated. Thanks!
20 December 2022 892 0 View
Is it possible to raise antibodies using GST-tagged chimeric proteins? And also using only GST protein as a control?
12 December 2022 4,058 3 View
In many books and papers, they used to use the complex form of the channel effect response. However, in reality, the signal is real values and also the channel. Is it correct to use the following...
07 December 2022 9,982 3 View
Hello all, I am facing an annoying problem wherein I am attempting to remove my GST tag from my (glutathione eluted) chimeric protein using a Thrombin Cleavage kit (Biotinylated Thrombin), but...
06 December 2022 3,819 3 View
We try to titer our M13 phage using double agar layer plates, but we couldn’t see any plaque after plate incubation. However, colonies can be observed on the plate containing antibiotic...
03 December 2022 7,535 0 View