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Questions related to Vectorization
What could be the possible reasons?
01 August 2023 3,486 0 View
Hi everyone does adding Triton to the bacterial culture medium prevents the formation of inclusion bodies and lead to an increase in protein solubility in the cytoplasm?
29 July 2023 4,222 3 View
Are they both same or are they different?
26 July 2023 5,773 3 View
Hello researchers I'd like to know what other method can be used to assemble 3 different DNA sequences, apart from the recombination method in saccharomyces cerevisiae, Gibson assembly, and PCR...
17 July 2023 7,423 1 View
Inbred lines can capture and enhance the efficiency of heterosis breeding. Development of inbred lines in a perennial crop takes many years and requires experimental space.
15 July 2023 4,940 2 View
Please share the protocols for transformation in diploid strain of fission yeast
07 July 2023 6,909 0 View
I am trying to use double selection marker, G418/kanR and BleoR in pPICZalpha plasmid. I am constructing the plasmid with both antibiotic resistance gene and clone it into E. coli Top10. However,...
05 July 2023 251 5 View
Hello fellow researchers!! This question is based on a very unfortunate dry ice shipment incident. Recently I booked a dry ice shipment for delivering Synthego's chemically modified gRNAs...
23 June 2023 939 0 View
Thank you.
18 June 2023 5,088 0 View
https://www.surveymonkey.com/r/5KQP3D6 For all the experts in green neighbourhoods certification systems, urban planning, or sustainable development I'm currently conducting an expert-based AHP...
15 June 2023 9,694 2 View
I am trying to transfect an sh-rna in my mammalian cells using lipofectamine 2000 reagent but every time transfection does not completely. Im using 1.5 ug of dna 5ul of lipofectamine and 200ul of...
13 June 2023 8,582 0 View
Hello everyone, I have a recombinant plasmid always getting overlapped peaks when sequencing. The sequencing primer is located on the backbone of the vector, tens of nts ahead the cloning site,...
08 June 2023 8,592 2 View
Dear community, Does anyone has experiences with bacterial cell free system? if any bacterial promoter can exhibit strong strength as high as T7 promoter, or quite similar? Thanks!
02 June 2023 1,678 0 View
after adding Isopropanol, and centrifuging, I’m not seeing visible white pellet at the bottom of the EP tube, is that means I proceed wrong ? please need some help !
25 May 2023 1,497 3 View
Im working on avian leukosis virus subject. therefore I want to collect some data about "updates on immunity and diagnostic tools" thanks for your attention and help.
22 May 2023 9,177 0 View
primers for detection of mutation
19 May 2023 2,601 2 View
Hello fellow researchers! We are trying to transform Arabidopsis with pCAMBIA2022, but we realized that the Chloramphenicol resistance inside the vector will not be compatible with the Agro...
19 May 2023 3,812 1 View
Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...
17 May 2023 832 3 View
/profile/Christian-Valentin contains 7 publications /profile/Christian-Valentin-4 contains 334 publications
07 May 2023 5,442 1 View
I had performed ligation reaction at 4 °C (overnight) and 23 °C (4 hours) and when i transformed my ligation mixture using DH5-alpha competent cells, colonies appeared only with 4°C ligation...
26 April 2023 4,713 0 View
I'm trying to build a cosmid for B. subtilis, but there are no commercially available cosmids with a gram-positive ori already installed. I was thinking of obtaining a commercial cosmid (SuperCos...
20 April 2023 4,549 0 View
I am currently undergoing my end of year research project which is testing if DNA can be found in a secondary transfer when multiple transfers have occurred, one of my replicates have a Ct value...
14 April 2023 6,199 4 View
I want download bibliometric data for research purposes.
01 April 2023 8,371 1 View
As asked in the question, i just coupled SWMM hydraulic part to a hydrodynamic model, now i am testing the coupled model with some simple cases, which shows plausible results. But i don not have...
29 March 2023 3,100 1 View