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Dear all I faced a confusing task. I used pimer LP and RP download by http://signal.salk.edu/tdnaprimers.2.html website for verifying the T-DNA insertion of WiscDsLoxHs. The PCR results was...
02 December 2022 5,247 2 View
Hi everyone, I'm working on a transcription factor which binds as a dimer its target DNA, composed by two palindromic half-sites (5'-TTGGCnnnnnGCCAA-3'). Talking about Electrophoretic Mobility...
25 November 2022 837 1 View
I tried 1XTE procedure but its not working and I get the Lawn in my plate.
23 November 2022 3,832 2 View
Currently, I am studying a project about electric charge station location selection. I can't find a trial version of ArcGIS Pro for Mac and I don't know if ArcGIS online has the capability to...
23 November 2022 3,566 4 View
hello if i have a signal for example x=[1 0 1 0 0 1] and i want to convolve it with a filter that have for example three points and i want the result to be the signal without the leakage how can...
20 November 2022 4,129 3 View
Hi, I am working with TRBO-G vector with the size of 11.2kb, when i am doing plasmid isolation, it showing single band structure in agarose gel. can anyone help me to solve the problem with...
18 November 2022 5,244 1 View
I'd like to konw the difference of the SVD method and the Newton method in solving geophysical problems. Whether they are both a tool for optimizing the overall objective function, but it seems...
02 November 2022 6,682 1 View
I like to do my Ph.D in mechanical engineering. My first option is Deemed to be University in India. And the Deemed to be university is approved by the University grands commission. But some...
11 October 2022 8,004 5 View
what kind of methods, advices or exercise which can help readers to increase their confident while they perform infront of audience do you know?
07 October 2022 5,974 3 View
I am encountering some challenges with single channel and multichannel EEG DMD MATLAB code. Can someone help please? clc clearvars close all %% [Signal, Fs] = ..................................
21 September 2022 4,026 0 View
Hello. In real moment I'm designing the CPA system for my Ti:Sa laser. I have only cw diode module for pumping (10 W). How can I evaluate amplification opportunity in 'regen'. I want to gain from...
19 September 2022 5,426 1 View
Question for anyone well versed in the micropipette puller universe. My Sutter Instrument Model P-1000 Flaming/Brown Micropipette Puller out of no where indicates an error upon startup. It says "A...
13 September 2022 8,295 0 View
I would like to assess transcriptome upregulation or down regulation in my plants between treated and control. I know there is the TruSeq RNA Sample Prep Kit v2 - Set A for easy mRNA isolation and...
03 August 2022 6,841 2 View
What happen If rotor of induction have only rotor bars and without laminated iron core ?
02 August 2022 1,606 2 View
We have a server installed in our department with the following configuration. Dell PowerEdge R740 2 x Intel Xeon Gold 6128 12/24 cores/threads Core frequency: 3.40 GHz 256 GB RAM SSD RAID1 480GB...
29 June 2022 5,038 1 View
Looking for the calculation or methods to determine the inertia, J (kgm^2) and friction factor, B (Nms) for 3phase induction motor.. Thank you!
11 June 2022 8,590 0 View
I use the bitcoin price and other block chains data to do the Johansen Cointegration test. The result show that they have two Cointegration. Then, I construct the VECM, but I don't know how to...
07 June 2022 4,477 3 View
Hi everybody, I purified a recombinant Pathogenesis Related protein belonging to class 10 (PR-10) and I want to test if the enzyme has RNase activity. I read that is possible to test that activity...
13 May 2022 3,610 2 View
I'm currently performing Agrobacterium transformations of immature wheat embryos for a CRISPR-Cas9 project. I do not have any colleagues with experience of working with wheat transformations and I...
26 April 2022 1,264 3 View
We know that there's T equivalent circuit and inverse Γ-equivalent circuit for 3 phase induction motor(IM). When the IM is operating under normal situation, the windings are symmetrical, so all 3...
13 April 2022 8,126 4 View
I want to know how can I calculate absorption by poynting vector and electric field and I need integrate for that.
03 April 2022 6,091 0 View
I have a genomic DNA library digested with BamHI enzyme and ligated into a pUC19 vector. I am planning to amplify specific genes. Is it necessary to first add BamHI enzymes to release the genes...
21 March 2022 9,228 3 View
Does pcDNA3.1 vector not work for HEK293T cells?
18 March 2022 3,635 2 View
Hi Dear all, I want to calculate the normal vectors of the Tyrosine amino acid ring and the chromophore rings. In that way, I want to calculate the angle between these vectors and distances. I...
16 March 2022 5,741 1 View