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Questions related to Vectorization
In the lab we used to use Matrigel (10 µl) for coating of 8-well Ibidi chambers and added cells with DMEM (Gibco 11965092) + 5% Matrigel. This worked fine but if we wanted to do imaging you needed...
02 March 2022 950 0 View
Hello everyone, I'm currerntly working with a Cas12a vector, which contains a cutting site for Eco31i and a 19 bp direct repeat, which is necessary for the Cas12a to function. After the...
22 February 2022 3,049 1 View
Hi, I have tried to verify the function of one enzyme while my target protein is insoluble in E.coli expression system , I have two thoughts: 1. trying the yeast expression system 2. try to find...
22 February 2022 3,141 3 View
I'm recently making research on induction motor parameter identification and found a research matching my purpose, but I don't know how to build this "ABCabc model of an induction motor", which is...
10 February 2022 6,534 5 View
I would like to know the specific reason why the angle with respect to the positive direction of the x-axis is used when calculating for the components of a vector.
07 February 2022 9,981 2 View
I have two complex vectors, let's say a and b. How to compute cosine similarity between a and b using MATLAB?
26 January 2022 2,239 4 View
thanks
23 January 2022 551 3 View
So I have been trying to construct a phylogenetic tree based on a collection of ITS sequences of a library of fungal isolates I cultured. However, after constructing it on MEGAX with my aligned...
22 January 2022 5,891 3 View
Kindly explain the mechanism behind it, I shall be very thankful to you
21 January 2022 2,868 0 View
Hi! I am characterising the distribution of cells and their repartition on a structure in confocal images. I have measured the orientation and distance of the cells from the center point of the...
11 January 2022 1,111 1 View
I’m looking for some help troubleshooting my cloning process. I’m trying to ligate a shRNA into a plasmid (pWALIUM20, ~10Kbp) but have been unsuccessful many times. I’m using a double digest...
11 January 2022 6,377 2 View
Looking to use Cas9 for research in a startup (possibly lentiviral), and would like input on what are the legal requirements and how to obtain Cas9 expression vectors (Cas13 is available from...
09 January 2022 6,389 3 View
HI. if I have 7 industry dummy variables and another dummy for listing status all being dichotomous in my model. it results multicollineartiy issues when including all the dummies in the...
04 January 2022 2,719 1 View
When I inversion the component of magnetic vector , My、Mz shows a fit result , but Mx often shows positive and negative associated results or tiny value. So when I try to make a profile about...
13 December 2021 9,430 1 View
I'm doing a subcloning experiment with a pTNT vector and I treated it and my inserts (engineered with RE recognition sites) with EcoRI and KpnI, then incubated the digested vector with alkaline...
10 December 2021 3,723 3 View
Hi great Researchers, We are planning for animal trial where we will implant a PDMS based medical device for 2-3 weeks. I was wondering, can I use normal lab Oven (...
08 December 2021 5,912 3 View
hello to all.. I want to calculate the sum of the x component of velocity, the y component of velocity, and then the magnitude of velocity ... which commands can I use...
07 December 2021 3,982 1 View
I have estimated a VECM model in EViews and using Johansen test I obtained that there were cointegration vectors. The output for short-run equation of VECOM contains two cointegration vectors, I...
06 December 2021 8,590 8 View
This is a fundamental question about basic phage library construction. I know that people can construct library by either phage vector (eg. M13KE) or phagemid+helperphage. Based on my...
04 December 2021 8,654 4 View
I'm wondering whether it's possible to do a Kruskal-Wallis test on difference scores, since I can't seem to find an example of this online. I think it would make sense conceptually, but I would...
02 December 2021 2,104 5 View
I would like to ask a question. I have performed many measurements on my samples with the free space method using the vector analyzer: N5230A PNA-L Network Analyzer, 2-ports Now I would like to...
01 December 2021 7,221 13 View
I am working on BEM. I can compute its stiffness matrix A. (AX=B). X= surface potentials and their normal derivatives B= source vector I want to compute leadfield matrix and I don't know how I...
01 December 2021 9,479 3 View
Hi All, I have a protein sequence cloned in pET21+ vector. Below is the part of the sequence starting from T7 promoter to the ATG (of my...
30 November 2021 4,936 3 View
I went through the book "Surface Plasmons on Smooth and rough surface and on Gratings by Raether." If you look at the equations 2.4, 2.5, and 2.6 in the link...
29 November 2021 3,047 4 View