Contact experts in Vectorization to get answers
1,052 views 884 posts
Questions related to Vectorization
Dear I would like to plot the FFT while I am having only a table of two vectors times and amplitude! is that possible? could someone guide me!
21 April 2021 2,627 5 View
I am looking for a software (ideally free) which can simulate polarized ATR-FTIR spectra of a stretched macromolecule, that has a specific orientation with respect to the electric field vector of...
21 April 2021 4,638 3 View
Dear researchers, The attached images are from the leaves of transformed plants by empty vector pCAMBIA1302 (GFP), so the images are ok and can use for a manuscript? Your comments are...
15 April 2021 5,999 11 View
I am working on a new smart ball concept. I have the equations that govern the trajectory of a spherical ball in motion. To calculate this i have two variables, Velocity vector and Spin vector. I...
02 April 2021 7,504 3 View
Hello everyone, I'm a PhD student producing recombinant proteins amplified from genomic DNA and I'm having issues with cloning. I'll explain briefly. I'm amplifying DNA sequences from genomic...
18 March 2021 2,827 4 View
If extra copy of restriction site need to be added for digestion of a vector with Bsa1, how such oligos are designed ?.....What should be the sequence composition ? Could anyone please help me to...
18 March 2021 4,403 2 View
I have an image from the microscope, which i can or not stack, and i want to do a xz vertical section using ImageJ/Fiji to see if i have a localization in the membrane or not. I know it is...
16 March 2021 828 1 View
Hi everyone, I am trying to get a 3xflag tag (around 100bps) onto the end of a sequence of interest. I have the vector (3kbp), with the sequence already in it and an EcoRV site where I want to...
12 March 2021 6,917 2 View
Hi, I am trying to order membrane protein synthesysed gene [about 1200 bp long] to do Gibson assembly by myself because it looks like the cost for ready to express clone in using my vector is...
10 March 2021 3,128 3 View
Hi, I received a frozen vector (rAAV) solution and thawed it to aliquot. I was unaware that I had to thaw the solution on wet ice and thawed it by rubbing the bottle in between my hands. Big...
08 March 2021 6,212 4 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
01 March 2021 5,108 2 View
1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...
21 February 2021 2,594 1 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
16 February 2021 4,530 3 View
Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...
16 February 2021 9,815 6 View
I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...
05 February 2021 638 1 View
I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...
24 January 2021 2,936 3 View
I want to plot the distribution curve of dipole moment as a function of angles between dipole vector and solid surface in the presence of external electric field. My system is consisting a...
18 January 2021 1,706 4 View
I want to perform gateway cloning (from LR reaction onwards) , how could i get the LR sequences to attach them with my sequences in donor and insert. Give me your suggestion, i will be thankful...
14 January 2021 3,629 3 View
Hi Everyone, I am a beginner at eukaryotic(HEK293T) protein expression. I am trying to purify a HIS tagged 200 kDa viral polyprotein using pCDNA3.1 vector. I am not sure which lysis buffer should...
12 January 2021 2,137 3 View
Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...
07 January 2021 1,135 3 View
I'm using MorphoJ, and the software gives me the eigen values but it doesn't give you the eigen vector. For each landmark it gives you a x and y value for each PC but I'm looking for a value that...
22 December 2020 3,663 3 View
I want to know whether a luenberger observer or a modified form of it be effective for estimating the states of a system of the following forms: 1. x_dot = f(x,u,d); y = x 2. x_dot = f(x,u); y =...
21 December 2020 1,622 4 View
Please, I am trying to do a project work using logistic regression. I need to test for multicollinearity for the independent variables using R. I installed mctest and multiColl package already....
19 December 2020 2,690 3 View
Can anybody help in understanding how in a MIMO system with large (ideally infinite) number of transmit/receive antennas and poor scattering (e.g at mmWave), the transmit/receive array response...
18 December 2020 7,078 4 View