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Questions related to Vectorization
Hi everyone, I am facing an issue with running the denaturing Urea-PAGE to visualize the digested fragment of the Oligos (the first labeled oligos are Cy2, and the second one is Cy5). First...
19 January 2023 6,504 0 View
I found several parameterless algorithms where hyper parameter tuning is not required. Which one can be considered the best in terms of robustness and suitable for solving non linear engineering...
19 January 2023 2,965 2 View
There are several global and freely available digital elevation models (e.g. SRTM, GDEM, ALOS...). However, they all have one thing in common and that is that these datasets are getting quite out...
16 January 2023 1,762 8 View
Are they non culturable/ lose their viability due to loss of their natural niche?
05 January 2023 5,103 3 View
Can you recommend peer-reviewed articles or papers which use the AHP survey to a set of experts? Your responses will be highly appreciated. Thanks!
20 December 2022 1,029 0 View
Is it possible to raise antibodies using GST-tagged chimeric proteins? And also using only GST protein as a control?
12 December 2022 4,164 3 View
In many books and papers, they used to use the complex form of the channel effect response. However, in reality, the signal is real values and also the channel. Is it correct to use the following...
07 December 2022 10,141 3 View
In Vaidya spacetime, for linear time -depended mass function, there is a conformal Killing vector. One can do coordinate transformation and this conformal Killing vector becomes (1,0,0,0). This...
07 December 2022 2,305 0 View
Hello all, I am facing an annoying problem wherein I am attempting to remove my GST tag from my (glutathione eluted) chimeric protein using a Thrombin Cleavage kit (Biotinylated Thrombin), but...
06 December 2022 3,965 3 View
We try to titer our M13 phage using double agar layer plates, but we couldn’t see any plaque after plate incubation. However, colonies can be observed on the plate containing antibiotic...
03 December 2022 7,647 0 View
Dear all I faced a confusing task. I used pimer LP and RP download by http://signal.salk.edu/tdnaprimers.2.html website for verifying the T-DNA insertion of WiscDsLoxHs. The PCR results was...
02 December 2022 5,429 2 View
how to use Element type STRI3 in Abaqus ?
26 November 2022 9,628 2 View
Hi everyone, I'm working on a transcription factor which binds as a dimer its target DNA, composed by two palindromic half-sites (5'-TTGGCnnnnnGCCAA-3'). Talking about Electrophoretic Mobility...
25 November 2022 943 1 View
I tried 1XTE procedure but its not working and I get the Lawn in my plate.
23 November 2022 3,985 2 View
Currently, I am studying a project about electric charge station location selection. I can't find a trial version of ArcGIS Pro for Mac and I don't know if ArcGIS online has the capability to...
23 November 2022 3,704 4 View
hello if i have a signal for example x=[1 0 1 0 0 1] and i want to convolve it with a filter that have for example three points and i want the result to be the signal without the leakage how can...
20 November 2022 4,277 3 View
Hi, I am working with TRBO-G vector with the size of 11.2kb, when i am doing plasmid isolation, it showing single band structure in agarose gel. can anyone help me to solve the problem with...
18 November 2022 5,385 1 View
I'd like to konw the difference of the SVD method and the Newton method in solving geophysical problems. Whether they are both a tool for optimizing the overall objective function, but it seems...
02 November 2022 6,843 1 View
accurately, i wanna know how to design a transmissive metamaterial cell that produces a wide range of phase differences?
22 October 2022 9,418 1 View
I like to do my Ph.D in mechanical engineering. My first option is Deemed to be University in India. And the Deemed to be university is approved by the University grands commission. But some...
11 October 2022 8,167 5 View
what kind of methods, advices or exercise which can help readers to increase their confident while they perform infront of audience do you know?
07 October 2022 6,114 3 View
I am encountering some challenges with single channel and multichannel EEG DMD MATLAB code. Can someone help please? clc clearvars close all %% [Signal, Fs] = ..................................
21 September 2022 4,144 0 View
Hello. In real moment I'm designing the CPA system for my Ti:Sa laser. I have only cw diode module for pumping (10 W). How can I evaluate amplification opportunity in 'regen'. I want to gain from...
19 September 2022 5,575 1 View
Question for anyone well versed in the micropipette puller universe. My Sutter Instrument Model P-1000 Flaming/Brown Micropipette Puller out of no where indicates an error upon startup. It says "A...
13 September 2022 8,428 0 View