Hello, I would like to ask you for help. I am trying to find genotyping protocol for roy a9 and slc45a2 b4 zebrafish mutants. Does anyone know them? Thanks.
Hello, I have conducted a multiple regression with two predictor variables and would like to find out whether the difference between the standardized regression coefficients is significant....
22 June 2020 8,788 11 View
I have a data-set with good and malicious files. Labelled as 0 and 1. In the data-set there are mor malicious files - like 3000 vs 800 good files. Nevertheless I am getting good results with...
27 April 2020 3,752 14 View
I have a few thousand files and each file has multiple "issues" after being deconstructed. They are labelled 0 or 1 for good or malicious. I think these "issues" can be a feature to determine if...
02 April 2020 2,941 2 View
2x2x2 Design
26 November 2019 4,808 3 View
Dear all, we need a procedure to rapid drying plant materials from field or greenhouse experiments to rapidly denaturing enzymes, kills micro-organism and stabilizes the tissues. On one hand we...
31 October 2018 5,942 9 View
At the moment we hear a lot about artificial intelligence and machine learning. It is an awesome development, unimaginable completely or partially, now. How and when humans will gain the benefits...
19 September 2018 801 25 View
21 July 2018 2,931 3 View
I am preparing samples for NGS, RNA sequencing. I isolate total RNA from B. subtilis, deplete it from rRNA and then make DNA libraries. Every step is quality-checked using BioAnalyser. And in the...
08 April 2018 7,871 6 View
Does anybody have any experience with cells detaching from Lab-Teks and how to deal with it? In one of our experiments we are not able to use gelatin or anything else. In other experiment we are...
13 September 2017 4,777 1 View
19 June 2017 5,329 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...
23 February 2021 1,934 1 View
Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...
18 February 2021 8,719 1 View
I need to do novel tank test and light/dark test for zebrafish. So I need a tracking software for tracking in these tests. Is there any free softwares available for tracking of zebrafish ?
16 February 2021 1,541 1 View
Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...
07 February 2021 891 3 View
Our in situs (alkaline phosphatase based) have stopped working. Probes are fine, but or homemade hybridization buffer seems to be the issue. Using the same supplier of deionized formaminde as...
03 February 2021 8,540 2 View
02 February 2021 8,877 3 View
Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...
02 February 2021 3,861 3 View