I would like to ask the following, if I need to grow rhizobia (Bradyrhizobium diazoefficiens) on high nitrogen medium and low nitrogen medium, what is the formula of high nitrogen medium and low nitrogen medium? Or is there any papers recommendation?
The nitrogen medium used for cultivating Bradyrhizobium diazoefficiens, a nitrogen-fixing bacteria commonly associated with soybeans, is generally referred to as the YEM medium, which stands for Yeast Extract Mannitol medium. This medium provides the necessary nutrients and environment for the growth and proliferation of Bradyrhizobium diazoefficiens.
The composition of the YEM medium typically includes:
- Yeast Extract: 0.4%
- Mannitol:0.4%
- Calcium chloride:0.05%
- Magnesium sulfate:0.02%
- Potassium dihydrogen phosphate:0.1%
- Agar:1.5-2% (if solid medium is needed)
The pH of the medium is adjusted to around 6.8 before sterilization. This formula provides essential nutrients and a suitable environment for the growth and maintenance of Bradyrhizobium diazoefficiens cultures, facilitating their study and utilization in agricultural applications, particularly in enhancing nitrogen fixation in leguminous crops like soybeans.
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