I checked a lot of software like DIANA Tools, Sponge Scan, Mirtat Target Scan, etc., but unfortunately, I did not get any results.
I have an ODE function with 5 variables that is complicated to solve theoretically. Therefore, I solved it numerically and found the equilibrium point (values for the variables). Is there an easy...
01 July 2024 5,223 3 View
The price of gold chloride salt is unfortunately very high.
08 June 2024 1,468 0 View
Greetings, recently I have acquired the pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector (V012884). I would like to know whether the gene of interest's expression is regulated by the CMV promoter or not,...
22 May 2024 8,127 2 View
I am working with a flowering mutant, so the transformation is not so easy, I need to repeat it every 7 days, but I was wondering what will happened to the flowers if I dip them again in...
01 May 2024 7,140 2 View
I have seen type of MOF powder perfectly 7:3 Ni/Fe by solvothermal method how ever when I put Ni foam in the autoclave and the same I can not have the appropriate MOF coating on that foam. I...
16 April 2024 6,554 0 View
I cloned 3HR and 5HR of p.falciparum on a plasmid and the next step is gRNA cloning. I did colony PCR for verification it is perfect but the thing is after taking the toothpeak of the colony in...
12 March 2024 5,716 2 View
I ran restriction digestion on my plasmid just to verify my inserts and send for sequencing. my backbone plasmid is PL6 with 9843 bp containing a 3HR (877bp) and 5HR (782). My enzymes for 3HR is...
07 February 2024 8,015 4 View
Dear researchers and experts, I've been investigating different testing methods for refractory bricks with special shapes. There are a few methods and standards for testing refractories; I wonder...
07 January 2024 830 3 View
I need a research project on pediatric ALL please help me
25 October 2023 2,528 4 View
Hello everyone, I have 2 BET diagrams for precipitated silica, and tests were held under the N2 atmosphere. There isn't any sign of isotherm in none of them. I don't know how I can explain it. I...
21 August 2023 3,791 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,080 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,537 5 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,401 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,618 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 806 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,364 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
Molecular docking software/ websites?
02 August 2024 8,703 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 223 2 View