The DNA polymerase fills in the gap due to overhangs (using a pair of primers carrying overhangs) during the 2nd round of replication in PCR. Does those gaps actually get filled or they have overhang of lesser number of bases. Shall we need blunting enzymes to treat the amplicon to ensure the presence of blunt end?
Paul Rutland
The polymerase will fill in the overhang on both strands during the pcr but some enzymes will also add an extra A base at the end of the strands ( as used in TA cloning) so you may need to generate blunt ends if you are blunt end cloning depending on what polymerase you are using in the pcr
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