Hi everyone,

I’m having trouble maintaining the viability of my SVG p12 Fetal Glial Cells in culture. They don’t survive for more than two-three days, and once they reach 30% confluency, they start detaching and dying.

The cells are cultured in EMEM medium supplemented with 10% FBS and 1% Pen/Strep.

Has anyone experienced a similar issue or found a solution to improve their survival? Any advice would be greatly appreciated!

Thanks in advance!