Hi. I need help to ID this white colony (4-7 days old at room temp). If more than 7 days of incubation, it turns green on top of the PDA agar and yellow at the bottom.
Kouadri Mohamed El Amine Apologize. I posted the wrong image, but I've already corrected it. Thank you!
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...
27 January 2021 8,702 3 View
Hi there, I am currently using Alfa Aesar fused quartz slides for my UV-vis and PL measurements of TiO2, but it seems that their slides emit in the UV around the same wavelength region where I...
19 January 2021 438 3 View
I have never conducted a meta analysis before, so I was wondering how to approach it. Let's say I wanted to look a how a specific metric has evolved over the past 20 years, for example the...
08 November 2020 8,663 2 View
Hi, I was wondering what the longest period of time anyone has done an experiment involving daily oral gavage? I have a model that takes 6 months to fully develop and need to begin treatment at...
05 November 2020 6,243 3 View
This is really two questions. I'm trying to convert P-S bonds into P-N bonds using Ag+ to pull off the sulfur, but when attempting the reaction in THF, either the silver sulfide was dissolved in...
17 June 2020 9,066 8 View
Can I know what is the sampling rate of ifft() command which is a build in function in matlab?
01 June 2020 7,937 12 View
I am dealing with ' Optimal signal processing of frequency-stepped CW radar data' from the website of Optimal Signal Processing of Frequency-Stepped CW Radar Data I want to find the hidden...
27 May 2020 1,125 12 View
Looking at the various CoV genomes, there are peptides/small proteins, aside from ORF1/2 on the genome, of which functions are unknown. These could circulate and in theory, once in abundance,...
25 March 2020 5,948 5 View
Hi there, I'm looking to buy various photodiodes/solar cells for some experiments. The cells I'm looking for are InGaP, CZAS (and CZT) and amorphous silicon. Any pointers to where I could buy...
13 February 2020 2,938 5 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?
21 February 2021 7,783 12 View
Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...
16 February 2021 1,664 3 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...
14 February 2021 2,039 4 View
Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...
10 February 2021 6,104 1 View
I would appreciate if you answer my DBA research questionnaire. Thank you all in...
10 February 2021 1,990 3 View