How to solve spikes and peaks in interpolated raster surfaces with Kriging?

25 November 2020 9,657 4 View

Anyone used Presenilin 1 [C1C2] Antibody by GeneTex?

Hello, I'm performing WB analysis using the Antibody anti-Presenilin 1 [C1C2] (cat number GTX116016) of the GeneTex company. The datasheet specifies that this antibody recognizes the full-length...

03 August 2020 7,086 1 View

Long-term forest data?

Does anybody know if some datasets on trees health or growth time series are freely available for Europe? My goal would be to study the climate change between 1901 and 2019 and to compare it with...

30 July 2020 3,201 4 View

What micropollutants can I analyze on HPLC ?

Greetings, I am wondering which micropollutant I may be able to see with HPLC analysis (no tandem MS), for example diclofenac or ibuprofen. I would test on spiked water samples, so theoretically I...

03 June 2020 3,723 3 View

How to treat mammalian diel activity patterns as a continuous variable?

Does anyone have experience in treating/converting mammalian diel activity patterns (diurnal/nocturnal/crepuscular/cathemeral) as a continuous variable rather than a factorial one? I am thinking...

13 May 2020 9,562 2 View

How do you make your analysis scripts 'reviewable' when there is a lot of bulky code repititions?

Hey everyone! How do you make your code readable for other people when there are long chunks of code which you repeat a lot (e.g. the same fancy figure for 10 DVs)? My current solution: I...

12 February 2020 549 1 View

Parallel computing and graphic CPU (GCPU) usage in R, is it possible with ALL R packages?

Hi all, surfing the web I wasn't able to find a simple and effective answer to my question. In other words, I need to speed up a code I wrote using several packages such as raster, rgdal, biomod2...

13 December 2019 1,703 4 View

HPLC analysis - why do I have different area values for the same sample?

Good morning, I am currently doing some standards for my HPLC. My interest compound is butyric acid and I am injecting it in four different concentrations in order to build the standard and the...

15 August 2019 9,049 5 View

Any suggestion for a low volume reverse osmosis filtration system?

Dear all, I am looking for a reverse osmosis system to concentrate a sugar solution at laboratory scale. The volumes I am going to treat are quite small, 10 - 50 mL and therefore I am looking for...

10 June 2019 4,280 2 View

HPLC analysis for VFA - how to correctly do an HPLC run?

Hello everyone, I've been using an HPLC for VFA detection but I have little experience. I'm specifically looking for acetate, propionate, butyrrate, valerate, etc. . I'm working with a c18 column...

28 May 2019 4,152 7 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View