Small RNA

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Questions related to Small RNA

Stable plant miRNAa as qPCR normalizers for Taqman Advance protocol?

We are going to use custom TaqMan TM Advanced miRNA Assays for microRNA quantification experiments in plants. These assays are not compatible with the use of U6 snRNA as a reference internal...

11 November 2017 8,908 3 View

Agilent Bioanalyzer miRNAs of 35nt?

Hi, My sample contains miRNAs, but when I use the small RNA chip (Bioanalyzer Agilent), I get a peak at around 35 nt instead of 22 nt? I'm pretty sure that there are miRNAs in there; any...

11 November 2017 7,671 4 View

this is query regarding small RNA RNAseq (miRNA and piRNA) using NEB kit. i am putting my bioanalyser electropherogram ?

need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...

09 November 2017 7,849 3 View

Stable plant miRNAa as qPCR normalizers for Taqman Advance protocol?

We are going to use custom TaqMan TM Advanced miRNA Assays for microRNA quantification experiments in plants. These assays are not compatible with the use of U6 snRNA as a reference internal...

03 November 2017 4,597 2 View

How about the performance of BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for small-RNAs sequencing?

Hi everybody. As I am at the critical step of my project, I wonder which sequencing platform may results in more quantified and valid small -RNA Seq data. Today, the Illumina series are the...

10 October 2017 7,987 4 View

Are rep.s needed for small-RNA/RNA seq libraries?

In more than several papers studing small-RNA/RNA seq I can see for each treatment just one library has been prepared that consists of e.g. 12 pooled biological RNA samples, and no replication...

10 October 2017 6,153 2 View

Is "camel hump" in tape station a sign for adapter dimer?

Hi, I got these results in tape station (attached). The small RNA library size should be around 173bp, so I think that libraries 28, 31, 34, 36, 37 also have an adapter dimer.  I am going to...

10 October 2017 2,499 1 View

Is "camel hump" in tape station a sign for adapter dimer?

Hi, I got these results in tape station (attached). The small RNA library size should be around 173bp, so I think that libraries 28, 31, 34, 36, 37 also have an adapter dimer.  I am going to...

02 October 2017 554 1 View

Any tips for rodent sperm isolation/RNA extraction from sperm?

My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...

08 August 2017 1,662 2 View

Any tips for rodent sperm isolation/RNA extraction from sperm?

My lab is interested in extracting RNA from sperm (small RNAs specifically), and I believe we have the correct kit (Zymo Quick-RNA Micro-prep), but we already have a lot of trouble with extracting...

07 August 2017 8,795 1 View

How to Isolate small RNA and total protein from sample .

I have very less sample quantity and my purpose is to isolate both mi RNA ( along with total RNA) and protein from the same sample. Any available method or Kit that can be used to isolate both...

06 June 2017 637 2 View

Help with Qiagen miRNeasy micro kit to obtain small/large RNA fractions from drosophila heads for RNAseq?

Hi all, I'm trying to obtain small (200 nt) RNA fractions from drosophila heads (~20/sample) for RNA sequencing. I've been trying to use the miRNeasy micro kit from...

06 June 2017 4,286 4 View

(Bacterial) Small RNA Database

Hello Everyone. Does anyone suggest me the most recent and exhaustive Bacterial Small RNA Database? Data analysis: Mapping/alignment of RNA species smaller than microRNAs to mature microRNAsAim:...

05 May 2017 6,578 7 View

What type of non-coding RNA could be detected in RNASeq and miRNASeq?

Hi, I am interested in the non-coding RNA in peanut and their regulatory functions on mRNA.  I want to include as many as RNA classes and to study them in one experiment. I know that some poly-A...

05 May 2017 4,155 4 View

Can anyone please suggest a good protocol for extracting small RNAs from gram positive bacteria?

I am looking for a protocol for extracting small RNAs from gram positive bacteria..can anyone provide me with in detail protocol. thank u..

18 April 2017 6,041 1 View

Is a spacer sequence required when trying to fuse an amplified promoter to an shRNA sequence?

I am using the clontech adenoX in fusion (system 3) to generate an shRNA adenovirus due to the apparent simplicity of the protocol (no shuttle vector cloning required). For this approach, you...

04 April 2017 5,468 0 View

Can anyone please suggest a good protocol for extracting small RNAs from gram positive bacteria?

I am looking for a protocol for extracting small RNAs from gram positive bacteria..can anyone provide me with in detail protocol. thank u..

04 April 2017 6,269 1 View

Has anyone had trouble with Northern Blot reads using the non-radioactive DIG labeling system?

I am using a non-radiactive DIG labeling system of small RNAs on a Northern Blot. When I look at the image on c-DiGit, the picture is just black. When adjust the brightness and contrast, I have no...

23 January 2017 2,631 6 View

Problems with small RNA sequencing : low rna quantity, low small rna quantity, tools for measuring smallRNA, number of reads expected?

Dear respected members, I hope you can shed some light to problems i am currently having. A link to journals related to problem i am having is very much appreciated. I am sorry if my questions are...

14 January 2017 4,306 6 View

What can be the reason for getting sequence at different position from the expected one in 3' RACE?

I did 3' RACE to find 3' end for small RNA sequence in yeast using gene specific forward primer, and universal reverse primer. After sequencing I am getting sequence of my small RNA after poly A...

03 January 2017 6,511 2 View

What can be the reason for getting sequence at different position from the expected one in 3' RACE?

I did 3' RACE to find 3' end for small RNA sequence in yeast using gene specific forward primer, and universal reverse primer. After sequencing I am getting sequence of my small RNA after poly A...

01 January 2017 2,951 2 View

Problems with small RNA sequencing : low rna quantity, low small rna quantity, tools for measuring smallRNA, number of reads expected?

Dear respected members, I hope you can shed some light to problems i am currently having. A link to journals related to problem i am having is very much appreciated. I am sorry if my questions are...

01 January 2017 5,738 7 View

Has anyone had trouble with Northern Blot reads using the non-radioactive DIG labeling system?

I am using a non-radiactive DIG labeling system of small RNAs on a Northern Blot. When I look at the image on c-DiGit, the picture is just black. When adjust the brightness and contrast, I have no...

01 January 2017 3,269 7 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

27 December 2016 6,179 2 View

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