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Questions related to Small RNA
Hey everyone, I am trying to do a miRNA-Seq experiment, but as you all know, before doing so I need to prepare a library and when reading up on it and asking people from around the field I still...
29 July 2018 4,536 5 View
I have small RNA seq data of 2 different species in FASTA file. I want to search homologous as well as orthologous small RNAs from these 2 file. I tried to use BBmap software in linux operating...
06 June 2018 3,022 2 View
Hello I am trying to screen small RNAs from sequencing data. The genome of the species is available but not well annotated and no microRNAs have been identified yet. I am trying to find...
06 June 2018 5,482 2 View
Is it possible to get small RNAs from total RNA? if I precipitate total RNA with 70% ethanol for example, would the small RNAs be in the flowthrough on a miRNeasy column for example?
24 May 2018 2,638 2 View
Dear all, Few years ago, I used miRCURY Biofluids from Exiqon for extracting RNA from plasma for detecting miRNA by qPCR. However, this product is currently discontinued since Exiqon is now...
24 May 2018 7,129 4 View
Hi, I am preparing small RNA libraries for NGS, using the QIAseq kit of Qiagen Usually I get Canadian concentrations of around 1-10 ng/ul, but in the last batch all of the libraries were
05 May 2018 2,202 5 View
Hello everyone. Right now i am working on differential expression of miRNA using next generation sequencing. analysis post sequencing was done using "Small rna analysis" pipeline in a integrated...
23 April 2018 6,441 3 View
Hi, Does anyone have experience in RNA editing with CRISPR Cas9 method? Does this tool have application limited only to gene editing or I am wondering whether intergenic regions that code for...
20 April 2018 8,956 3 View
04 April 2018 3,177 4 View
04 April 2018 6,602 3 View
I'm interested in quantifying the total amounts of messenger RNAs and small RNAs present in leaf samples from different nutrient regimes. However I am aware that RNA is not a stable molecule and...
03 March 2018 7,813 1 View
I need to quantify the total miRNA in my sample in order to calculate the volume that should be used for microarray analysis. can I use small RNA chip/agilent in my case??
04 February 2018 1,721 3 View
My research specifically looks at the colorectal tumor's adjacent normal tissue and its small RNA (mainly miRNA) expression and its role as a prognostic marker. Currently I wish to interrogate...
22 January 2018 4,526 3 View
best possible approch for small RNA quantification
13 January 2018 3,725 3 View
01 January 2018 3,626 4 View
I am trying to extract total RNA from bacteria (Salmonella) using the mirVana kit. I am interested in studying the total trasncriptome, inlcuding small RNAs. But after extraction (I elute in...
22 December 2017 7,129 6 View
I want to do small RNA Next Generation Sequencing. To publish my paper in a high impact journal, do I need to take at least 2 replicates or pooling the sample without any replicate would work. I...
14 December 2017 9,923 2 View
12 December 2017 506 7 View
Hi there, I know that there are very good kits for miRNA isolation, but I want to use Trizol for this purpose. It is known that Trizol selectively loses GC-rich small RNAs when extracting RNA...
12 December 2017 5,378 12 View
12 December 2017 6,447 2 View
I checked the quality of total RNA of tomato tissues following Trizol extraction on non-denaturing gel. I am planning to submit my samples for small RNA-Sequencing. What caught my attention is...
26 November 2017 593 2 View
identification of small RNAs such as micrornas
11 November 2017 1,298 0 View
11 November 2017 2,668 2 View
need to find the real peaks for mi RNA and pi RNA before size selection procedure , i am not feeling confident enough for proper size selection step by amppure beads, imporantly dont want to go...
11 November 2017 799 3 View