Small RNA

Has anyone had experience using 5'polyphosphates? | ResearchGate

Hello. We are using 5'polyphosphates (LGC Biosearch Technologies) to remove uncapped RNAs. But have had little to no success with it. I have been using Lucigen protocol and afterwards GeneJET RNA...

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Small RNA loading dye recipe? | ResearchGate

I'm trying to replace my small RNA prestain ladder with a much cheaper tracking dye that I can make up myself. Has anyone any suggestions. I run 10% urea-PAGE gels for 75 mins at 300V. I tested...

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Using online tool (Eg Galaxy) any recommendations for IsomiRs differential expression pipeline? | ResearchGate

Any suggestions on preferred online tools for analysing small RNA seq data (isomiRs differential expressions) for those researchers who don't have any bioinformatics background.

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Sequencing of which RNA among small RNA or micro RNA is better in case of plants? | ResearchGate

I am working on differential expression and pathways involved in somatic embryogenesis of cotton plant. except Transcriptomics I want to go for noncoding RNA sequencing as well. Kindly suggest...

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How fragmentation of total RNA affects small RNAs (

Hello, I want to sequence enriched RNA (it will include both mRNAs and bacterial small non-coding RNAs (

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Is this degradation or 5S band? | ResearchGate

Hi there - I've been trying to optimise total RNA extraction from bacterial cultures, but I keep getting RIN = N/A on the Agilent Bioanalyser and a very sharp peak on the trace (about 25-30s). I...

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