Small RNA

Has anyone had experience using 5'polyphosphates? | ResearchGate

Hello. We are using 5'polyphosphates (LGC Biosearch Technologies) to remove uncapped RNAs. But have had little to no success with it. I have been using Lucigen protocol and afterwards GeneJET RNA...

01 January 1970 3,148 0 View

Small RNA loading dye recipe? | ResearchGate

I'm trying to replace my small RNA prestain ladder with a much cheaper tracking dye that I can make up myself. Has anyone any suggestions. I run 10% urea-PAGE gels for 75 mins at 300V. I tested...

01 January 1970 6,217 0 View

Using online tool (Eg Galaxy) any recommendations for IsomiRs differential expression pipeline? | ResearchGate

Any suggestions on preferred online tools for analysing small RNA seq data (isomiRs differential expressions) for those researchers who don't have any bioinformatics background.

01 January 1970 7,642 0 View

Sequencing of which RNA among small RNA or micro RNA is better in case of plants? | ResearchGate

I am working on differential expression and pathways involved in somatic embryogenesis of cotton plant. except Transcriptomics I want to go for noncoding RNA sequencing as well. Kindly suggest...

01 January 1970 7,968 0 View

How fragmentation of total RNA affects small RNAs (

Hello, I want to sequence enriched RNA (it will include both mRNAs and bacterial small non-coding RNAs (

01 January 1970 8,955 0 View

Is this degradation or 5S band? | ResearchGate

Hi there - I've been trying to optimise total RNA extraction from bacterial cultures, but I keep getting RIN = N/A on the Agilent Bioanalyser and a very sharp peak on the trace (about 25-30s). I...

01 January 1970 8,011 0 View

No title

01 January 1970 1,687 0 View

No title

01 January 1970 7,268 0 View

No title

01 January 1970 6,728 0 View