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Questions related to Small RNA
Hello! I plan to run cDNA intended for RNA-seq in a gel and excise the band of interest in order to get rid of the adaptor from the library prep. Can anyone suggest me an optimized size exclusion...
04 April 2016 4,722 1 View
Hello, In E.coli mutant strain, we observe a defect in 5S rRNA processing. I was wondering to know if there is another endogenous control for qRT-PCR. Thank you.
03 March 2016 5,815 3 View
Hi, As we know, the first nucleotide of microRNA is incline to be U and i would like to make a graph with the Analysis of 5′ nucleotide bias of small RNAs..
02 February 2016 2,509 1 View
genome scale metabolic reconstruction
02 February 2016 414 1 View
Hi everyone, I need to extract Campylobacter RNA while infecting amoeba cells. Does anyone know a suitable method or kit for this? My problem is to separate the bacterial RNA from the eukaryotic...
02 February 2016 4,082 1 View
To study functionality of miRNAs We are overexpressing miRNA-122 in the cells using lipofectamine. We want to cotransfect mimics as well as inhibitors. So how shall I go about it? Kindly share...
01 January 2016 7,829 3 View
I did a genomic DNA extraction Gram negative bacteria by Wizard® Genomic DNA Purification Kit. Samples were treated with RNase Sigma-Aldrich, but spots appear at the end of the gel. I suppose it...
01 January 2016 538 2 View
Hi I want to do NGS for microRNA presents in my exosomal samples obtained from cell culture. I have isolated exosomes followed by total RNA extraction. However, when I checked the quality on the...
12 December 2015 9,316 4 View
I think that U6 might be able to be used but I am not sure if it is secreted uniformally in exosomes. Would you recommend another housekeeping? Thanks
12 December 2015 9,230 8 View
I would like to validate miRNA NGS data by qRT-PCR. This theoretically could be done from total RNA, isolated small RNA (since that was used for sequencing library preparation) or the sequencing...
11 November 2015 9,584 4 View
Hi community, At the moment, I transfecting primary ventral horn mixed motoneuron cultures from E13 mouse embryos with small RNA fragments (30-45nt) to see their properties in protection against...
11 November 2015 9,334 1 View
I use Qiagen RNeasy Micro kit for small RNA purification. My starting material are eukaryotic cells, or material previously lysed in a different lysis buffer. Therefore, cell disruption is not a...
10 October 2015 5,440 3 View
Dear All, Could any of you suggest how to get to the function of small nucleolar RNAs ? I got tons of them after a small RNA sequencing experiment and would like to know their functions or...
09 September 2015 9,204 1 View
If anyone notice any pattern in viral genome while aligning small RNA sequencing data. Please share if anyone noticed it.
08 August 2015 7,078 2 View
Preferably from library generation to bioinformatic analyses. Turnaround time is also important.
07 July 2015 1,634 6 View
For example, I want to know how many reads are there in this experiment available at NCBI SRA database: ERX242531.Thanks in advance
07 July 2015 7,498 13 View
I am planning to make small RNA molecules and cap them. I want to check the ability of these m7G RNA's to eIF4E/eIF4F complex, whichever may be easier. Has anyone done anything like this before...
07 July 2015 9,765 2 View
Hello, Please tell me from where to download small RNA seq data of Idiopathic Pulmonary Fibrosis. Thanks
07 July 2015 885 2 View
I was preparing small RNA libraries for an NGS. For checking the results I did a cloning and test Sanger sequencing, where I ended up with the same sequence over and over again in two different...
06 June 2015 2,345 3 View
I am measuring levels on mirco RNAs in a murine myoblast cell line. So, I collected the cells and processed them for small RNA extraction using MirVana Kit. Upon estimating the concentration and...
18 May 2015 9,858 4 View
I want to perform two kind of experiments: a RNA-seq dedicated to mRNAs and lincRNAs, and a small RNA-seq to identify miRNAs. Both kit are from illumina, but the size of fragments to sequence will...
05 May 2015 10,107 3 View
05 May 2015 8,863 9 View
I did RNAs extraction from blood sample with directZol of Zymo, including small RNAs, but the quantity and quality was low, how I can improve that, thanks you very much for your...
05 May 2015 4,138 0 View
I did RNA extraction from blood sample with directZol de Zymo including small RNAs, but the quantity and quality was low, how I can improve that. Thanks you for your time and...
05 May 2015 9,317 3 View